Overview

  • Product name
  • Description
    Mouse polyclonal to PPT1
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human PPT1, amino acids 1-306 (NP_000301.1)

  • Positive control
    • Human liver tissue lysate, PPT1 transfected 293T cell lysate, Human salivary gland tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab89022 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
  • Application notes
    IHC-P: Use at a concentration of 3 µg/ml. Antigen retrieval is recommended Place sample in 1X citrate buffer (pH 6.0) and microwave at 750W for 20 minutes, cool sample
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Removes thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides during lysosomal degradation. Prefers acyl chain lengths of 14 to 18 carbons.
    • Involvement in disease
      Defects in PPT1 are the cause of neuronal ceroid lipofuscinosis type 1 (CLN1) [MIM:256730]. A form of neuronal ceroid lipofuscinosis with variable age at onset. Infantile, late-infantile, juvenile, and adult onset have been reported. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material, and clinically by seizures, dementia, visual loss, and/or cerebral atrophy. The lipopigment pattern seen most often in CLN1 is referred to as granular osmiophilic deposits (GROD).
    • Sequence similarities
      Belongs to the palmitoyl-protein thioesterase family.
    • Cellular localization
      Lysosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • Ceroid palmitoyl palmitoyl protein thioesterase 1 antibody
      • CLN1 antibody
      • EC 3.1.2.22 antibody
      • INCL antibody
      • Palmitoyl protein hydrolase 1 antibody
      • Palmitoyl protein thioesterase 1 antibody
      • Palmitoyl-protein hydrolase 1 antibody
      • Palmitoyl-protein thioesterase 1 antibody
      • PPT antibody
      • PPT-1 antibody
      • PPT1 antibody
      • PPT1_HUMAN antibody
      see all

    Anti-PPT1 antibody images

    • Anti-PPT1 antibody (ab89022) at 1 µg/ml + Human liver tissue lysate at 50 µg

      Predicted band size : 34 kDa
      Observed band size : 34 kDa
    • All lanes : Anti-PPT1 antibody (ab89022) at 1 µg/ml

      Lane 1 : PPT1 transfected 293T cell lysate
      Lane 2 : Non-transfected lysate

      Lysates/proteins at 25 µg per lane.


      Predicted band size : 34 kDa
      Observed band size : 34 kDa
    • ab89022 at 3µg/ml staining PPT1 in Human salivary gland by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue.

    References for Anti-PPT1 antibody (ab89022)

    This product has been referenced in:
    • Wang R  et al. Identification of palmitoyl protein thioesterase 1 in human THP1 monocytes and macrophages and characterization of unique biochemical activities for this enzyme. Biochemistry 52:7559-74 (2013). Read more (PubMed: 24083319) »

    See 1 Publication for this product

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    There are currently no Abreviews or Questions for ab89022.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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