Overview

  • Product nameAnti-PPTC7 antibody
    See all PPTC7 primary antibodies
  • Description
    Rabbit polyclonal to PPTC7
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    amino acids 118-215 (ILTTSYCELL QNKVPLLGSS TACIVVLDRT SHRLHTANLG DSGFLVVRGG EVVHRSDEQQ HYFNTPFQLS IAPPEAEGVV LSDSPDAADS TSFDVQL) of the Human PPTC7 protein (Q8NI37).

  • Positive control
    • Human colon tissue, RT-4, U-251 MG, Human Liver and Human Tonsil lysates.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122548 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

Anti-PPTC7 antibody images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli, plasma membrane and cytoplasm. Recommended concentration of ab122548 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122548, at 1:200, staining PPTC7 in Human colon tissue by immunohistochemistry [Paraffin Embedded Tissues (IHC-P)].
  • All lanes : Anti-PPTC7 antibody (ab122548) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human plasma cell lysate
    Lane 4 : Liver cell lysate
    Lane 5 : Tonsil cell lysate

References for Anti-PPTC7 antibody (ab122548)

ab122548 has not yet been referenced specifically in any publications.

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