Recombinant
RabMAb

Anti-PPWD1 antibody [EPR7439] (ab150356)

Overview

  • Product name
    Anti-PPWD1 antibody [EPR7439]
    See all PPWD1 primary antibodies
  • Description
    Rabbit monoclonal [EPR7439] to PPWD1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PPWD1 aa 1-100. The exact sequence is proprietary.

  • Positive control
    • HeLa, NIH3T3, and 293T cell lysates, HeLa cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR7439
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab150356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function
      Putative peptidylprolyl isomerase (PPIase). PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. May be involved in pre-mRNA splicing.
    • Sequence similarities
      Belongs to the cyclophilin-type PPIase family. PPIL1 subfamily.
      Contains 1 PPIase cyclophilin-type domain.
      Contains 4 WD repeats.
    • Cellular localization
      Nucleus. Associated with spliceosomal complexes.
    • Information by UniProt
    • Database links
    • Alternative names
      • 4632422M10Rik antibody
      • A330090G21Rik antibody
      • KIAA0073 antibody
      • OTTHUMP00000161887 antibody
      • Peptidylprolyl isomerase domain and WD repeat containing 1 antibody
      • Peptidylprolyl isomerase domain and WD repeat containing protein 1 antibody
      • Peptidylprolyl isomerase domain and WD repeat-containing protein 1 antibody
      • PPWD 1 antibody
      • Ppwd1 antibody
      • PPWD1_HUMAN antibody
      • RGD1310204 antibody
      • Spliceosome-associated cyclophilin antibody
      see all

    Images

    • All lanes : Anti-PPWD1 antibody [EPR7439] (ab150356) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : NIH3T3 cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 74 kDa
      Observed band size: 74 kDa

    • Flow Cytometrical analysis of permeabilized HeLa cells with ab150356 antibody at a dilution of 1/100 (red) or a rabbit IgG (negative) (green).

    References

    ab150356 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab150356.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up