General notesThe CMV promoter is covered under U. S. Patents 5,168,062 and 5,385,839 and its use is permitted for research purpose only.
Any other use of the CMV promoter requires a licence from the University of Iowa Research Foundation, 214 Technology Innovation Center, Iowa City, IA 52242.
Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Vector notespQM-NTag intron 5055 bp
REGION 1-444 polyA
GENE 444-644 SV40ori
GENE 2503-1643 Amp
REGION 3584-4189 CMV
REGION 4190-4259 tk leader
GENE 4275-4304 E2Tag
MCS 4305-4334 multiple cloning site
REGION 4336-5055 intron
The pQM-intron vectors are designed for expression and detection of E2- tagged fusion proteins in mammalian cells. The pQM-intron vectors contain rabbit beta-globin as an intron and provide E2Tag epitope tagging options, which allow E2Tag epitope tagging at either the N- or C-terminus of the protein. Mammalian expression is driven by the CMV immediate early promoter. The polyadenylation sequence provides signals required for termination of mammalian transcription and translation. pQM-NTag-intron vector allows cloning of E2Tag at N- terminus of the protein. pQM-CTag-intron vector allows cloning of E2Tag at C-terminus of the protein. Both pQM-NTag-intron and pQM-CTag-intron vectors are supplied with all three reading frames for easy cloning. The translational start sequence used in pQM-NTag-intron is a Kozak consensus sequence GCCATGG. pQM-CTag-intron vectors do not contain a translation start sequence. For more information on the E2 tag system see the datasheets for ab978, ab977, ab997 and ab1013.