Overview

  • Product nameAnti-PR3 antibody [WGM2] (FITC)
    See all PR3 primary antibodies
  • Description
    Mouse monoclonal [WGM2] to PR3 (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Tested applicationsSuitable for: WB, IHC-P, IHC-Fr, Flow Cyt, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    neutrophils (degranulated supernatant)human

  • General notes


    Blocks PR3 activity and partially inhibits the binding of human PR3-ANCA to PR3.

Properties

Applications

Our Abpromise guarantee covers the use of ab91182 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10. Predicted molecular weight: 28 kDa.
IHC-P 1/10.
IHC-Fr 1/10.
Flow Cyt Use 5µl for 106 cells. ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ELISA Use at an assay dependent concentration.

Target

  • FunctionPolymorphonuclear leukocyte serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema when administered by tracheal insufflation to hamsters.
  • Sequence similaritiesBelongs to the peptidase S1 family. Elastase subfamily.
    Contains 1 peptidase S1 domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACPA antibody
    • AGP 7 antibody
    • AGP7 antibody
    • AGP7 serine proteinase antibody
    • Azurophil Granule Protein 7 antibody
    • C ANCA antibody
    • C ANCA antigen antibody
    • C-ANCA antigen antibody
    • CANCA antibody
    • EC 3.4.21.76 antibody
    • Leukocyte proteinase 3 antibody
    • MBN antibody
    • MBT antibody
    • MBT WEGENER AUTOANTIGEN antibody
    • Myeloblastin antibody
    • Neutrophil proteinase 4 antibody
    • NP 4 antibody
    • NP-4 antibody
    • NP4 antibody
    • P29 antibody
    • PR 3 antibody
    • PR-3 antibody
    • PR3 antibody
    • Proteinase 3 antibody
    • Proteinase3 antibody
    • PRTN 3 antibody
    • Prtn3 antibody
    • PRTN3_HUMAN antibody
    • Serine proteinase neutrophil Wegener granulomatosis autoantigen antibody
    • Serine proteinase, neutrophil antibody
    • Wegener autoantigen antibody
    • Wegener granulomatosis autoantigen antibody
    see all

Anti-PR3 antibody [WGM2] (FITC) images

  • Overlay histogram showing peripheral blood lymphocytes cells stained with ab91182 (red line). The cells were fixed with 4% paraformaldehydel (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab91182, 0.5µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 (1µg/1x106 cells ). Acquisition of >5,000 events was performed.

References for Anti-PR3 antibody [WGM2] (FITC) (ab91182)

ab91182 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab91182.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"