Overview

  • Product name
    Anti-PR3 antibody [WGM2] (FITC)
    See all PR3 primary antibodies
  • Description
    Mouse monoclonal [WGM2] to PR3 (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Tested applications
    Suitable for: WB, IHC-P, IHC-Fr, Flow Cyt, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    neutrophils (degranulated supernatant)human

  • General notes


    Blocks PR3 activity and partially inhibits the binding of human PR3-ANCA to PR3.

Properties

Applications

Our Abpromise guarantee covers the use of ab91182 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10. Predicted molecular weight: 28 kDa.
IHC-P 1/10.
IHC-Fr 1/10.
Flow Cyt Use 5µl for 106 cells. ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ELISA Use at an assay dependent concentration.

Target

  • Function
    Polymorphonuclear leukocyte serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema when administered by tracheal insufflation to hamsters.
  • Sequence similarities
    Belongs to the peptidase S1 family. Elastase subfamily.
    Contains 1 peptidase S1 domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACPA antibody
    • AGP 7 antibody
    • AGP7 antibody
    • AGP7 serine proteinase antibody
    • Azurophil Granule Protein 7 antibody
    • C ANCA antibody
    • C ANCA antigen antibody
    • C-ANCA antigen antibody
    • CANCA antibody
    • EC 3.4.21.76 antibody
    • Leukocyte proteinase 3 antibody
    • MBN antibody
    • MBT antibody
    • MBT WEGENER AUTOANTIGEN antibody
    • Myeloblastin antibody
    • Neutrophil proteinase 4 antibody
    • NP 4 antibody
    • NP-4 antibody
    • NP4 antibody
    • P29 antibody
    • PR 3 antibody
    • PR-3 antibody
    • PR3 antibody
    • Proteinase 3 antibody
    • Proteinase3 antibody
    • PRTN 3 antibody
    • Prtn3 antibody
    • PRTN3_HUMAN antibody
    • Serine proteinase neutrophil Wegener granulomatosis autoantigen antibody
    • Serine proteinase, neutrophil antibody
    • Wegener autoantigen antibody
    • Wegener granulomatosis autoantigen antibody
    see all

Images

  • Overlay histogram showing peripheral blood lymphocytes cells stained with ab91182 (red line). The cells were fixed with 4% paraformaldehydel (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab91182, 0.5µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 (1µg/1x106 cells ). Acquisition of >5,000 events was performed.

References

ab91182 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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