PR3 overexpression 293T lysate (whole cell) (ab94310)

Overview

  • Product namePR3 overexpression 293T lysate (whole cell)
  • Description
    PR3 overexpression lysate
  • General notesab94310 is a 293T cell transfected lysate in which Human PR3 has been transiently over-expressed using a pCMV-PR3 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundFunction: Polymorphonuclear leukocyte serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema when administered by tracheal insufflation to hamsters. Similarity: Belongs to the peptidase S1 family. Elastase subfamily. Contains 1 peptidase S1 domain.

Associated products

Applications

Our Abpromise guarantee covers the use of ab94310 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

PR3 overexpression 293T lysate (whole cell) images

  • ab94310 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-PR3 antibody (ab88097) at 1/500 dilution

    Lane 1 : PR3 293T Transfected Lysate - Positive Control (ab94310)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugate at 1/2500 dilution

References for PR3 overexpression 293T lysate (whole cell) (ab94310)

ab94310 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"