Overview

  • Product nameAnti-PRAF1 antibody
    See all PRAF1 primary antibodies
  • Description
    Mouse polyclonal to PRAF1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length PRAF1 protein (Human)

  • Positive control
    • PRAF1 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab68293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).

Target

  • FunctionDNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF.
  • Sequence similaritiesBelongs to the eukaryotic RPA49/POLR1E RNA polymerase subunit family.
  • Cellular localizationNucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • A49 antibody
    • DNA directed RNA polymerase I subunit RPA49 antibody
    • DNA-directed RNA polymerase I subunit E antibody
    • DNA-directed RNA polymerase I subunit RPA49 antibody
    • FLJ 13390 antibody
    • FLJ13970 antibody
    • PAF53 antibody
    • POLR 1 E antibody
    • POLR1E antibody
    • Polymerase (RNA) I associated factor 1 antibody
    • Polymerase (RNA) I polypeptide E antibody
    • Polymerase (RNA) I polypeptide E, 53kDa antibody
    • PRAF1 antibody
    • RNA polymerase I associated factor 1 antibody
    • RNA polymerase I associated factor 53 antibody
    • RNA polymerase I polypeptide E antibody
    • RNA polymerase I subunit A49 antibody
    • RNA polymerase I-associated factor 1 antibody
    • RNA polymerase I-associated factor 53 antibody
    • RP11405L18.3 antibody
    • RPA49_HUMAN antibody
    see all

Anti-PRAF1 antibody images

  • All lanes : Anti-PRAF1 antibody (ab68293) at 1/500 dilution

    Lane 1 : PRAF1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 54 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

References for Anti-PRAF1 antibody (ab68293)

ab68293 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"