Anti-PRAK antibody (ab79433)


  • Product nameAnti-PRAK antibody
    See all PRAK primary antibodies
  • Description
    Rabbit polyclonal to PRAK
  • Specificityab79433 detects endogenous levels of total PRAK protein.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic non phosphopeptide derived from human PRAK around the phosphorylation site of threonine 182 (L-M-TP-P-Q).

  • Positive control
    • MCF7 cell extracts.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab79433 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab79433 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
ELISA 1/10000.


  • FunctionMediates stress-induced small heat shock protein 27 phosphorylation.
  • Tissue specificityExpressed ubiquitously.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Post-translational
    Phosphorylated on Thr-182; which is the regulatory phosphorylation site and is located on the T-loop/loop 12.
  • Cellular localizationCytoplasm. Nucleus. Also observed in the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • MAP kinase-activated protein kinase 5 antibody
    • MAPK-activated protein kinase 5 antibody
    • MAPK5_HUMAN antibody
    • MAPKAP kinase 5 antibody
    • MAPKAPK-5 antibody
    • MAPKAPK5 antibody
    • Mitogen activated protein kinase activated protein kinase 5 antibody
    • p38-regulated/activated protein kinase antibody
    • PRAK antibody
    see all

Anti-PRAK antibody images

  • All lanes : Anti-PRAK antibody (ab79433) at 1/500 dilution

    Lane 1 : MCF7 cell extracts
    Lane 2 : MCF7 cell extracts with immunising peptide at 10 µg

    Lysates/proteins at 10 µg per lane.

    Predicted band size : 54 kDa
    Observed band size : 54 kDa

References for Anti-PRAK antibody (ab79433)

ab79433 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (transformed murine B cells)
Loading amount 50 µg
Specification transformed murine B cells
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Mar 05 2012

Thank you for contacting Abcam.

I have returned the Abreview to you so that you can enter the 100% testing Discount code that you were sent,as the discount code does have to entered into the Abreview to become valid.

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I am very pleased to hear you would like to accept our offer and test ab79433 in mouse tissue. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To re...

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Thank you very much for your interest in ab79433.

We have not tested ab79433 in mouse tissue and only predict it will work based on sequence homology. As ab79433 has not been tested in mouse tissue, I can offer a discount off a future purcha...

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