Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling PRAK with ab93800 at 1/200 (1µg/ml). Detection: DAB.
Western blot - PRAK antibody (ab93800)
All lanes : Anti-PRAK antibody (ab93800) at 0.04 ��g/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique
Predicted band size : 54 kDa
Exposure time : 3 minutes
Immunoprecipitation - PRAK antibody (ab93800)
Detection of PRAK in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab93800 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.
References for Anti-PRAK antibody (ab93800)
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