Overview

  • Product nameAnti-PRAK antibody
    See all PRAK primary antibodies
  • Description
    Rabbit polyclonal to PRAK
  • Tested applicationsSuitable for: IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Bat
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 423-473 of Human PRAK (NP_620777.1)

  • Positive control
    • Whole cell lysate from HeLa and 293T cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab93800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 54 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • FunctionMediates stress-induced small heat shock protein 27 phosphorylation.
  • Tissue specificityExpressed ubiquitously.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated on Thr-182; which is the regulatory phosphorylation site and is located on the T-loop/loop 12.
  • Cellular localizationCytoplasm. Nucleus. Also observed in the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • MAP kinase-activated protein kinase 5 antibody
    • MAPK-activated protein kinase 5 antibody
    • MAPK5_HUMAN antibody
    • MAPKAP kinase 5 antibody
    • MAPKAPK-5 antibody
    • MAPKAPK5 antibody
    • Mitogen activated protein kinase activated protein kinase 5 antibody
    • p38-regulated/activated protein kinase antibody
    • PRAK antibody
    see all

Anti-PRAK antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling PRAK with ab93800 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-PRAK antibody (ab93800) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 54 kDa


    Exposure time : 3 minutes
  • Detection of PRAK in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab93800 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.

References for Anti-PRAK antibody (ab93800)

ab93800 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"