Overview

  • Product nameAnti-PRAK antibody
    See all PRAK primary antibodies
  • Description
    Rabbit polyclonal to PRAK
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Pig
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 197-467 of Human PRAK (AAH47284).

  • Positive control
    • WB: 293T and H1299 whole cell lysates; A431, HeLaS3, HepG2, MOLT4 and Raji cell lines ICC/IF: HeLa cells

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97332 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 54 kDa.
ICC/IF 1/100 - 1/200.

Target

  • FunctionMediates stress-induced small heat shock protein 27 phosphorylation.
  • Tissue specificityExpressed ubiquitously.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated on Thr-182; which is the regulatory phosphorylation site and is located on the T-loop/loop 12.
  • Cellular localizationCytoplasm. Nucleus. Also observed in the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • MAP kinase-activated protein kinase 5 antibody
    • MAPK-activated protein kinase 5 antibody
    • MAPK5_HUMAN antibody
    • MAPKAP kinase 5 antibody
    • MAPKAPK-5 antibody
    • MAPKAPK5 antibody
    • Mitogen activated protein kinase activated protein kinase 5 antibody
    • p38-regulated/activated protein kinase antibody
    • PRAK antibody
    see all

Anti-PRAK antibody images

  • All lanes : Anti-PRAK antibody (ab97332) at 1/500 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : H1299 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 54 kDa
  • ab97332, at a 1/200 dilution, staining PRAK in paraformaldehyde fixed HeLa cells by Immunofluorescence.
    Lower image is merged with DNA probe.

References for Anti-PRAK antibody (ab97332)

ab97332 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab97332.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"