Purification notesab59999 is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2 step procedure with the control and phosphorylated peptides. The phospho specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
ab59999 at 1/50 dilution staining PRAK in Human hepatocarcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue.
The primary antibody was peroxidase conjugated to the secondary antibody, followed by AEC staining.
References for Anti-PRAK (phospho S212) antibody (ab59999)
has not yet been referenced specifically in any publications.
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