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Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Preservative: 0.09% Sodium Azide
Concentration information loading...
Protein G purified
ab59999 is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2 step procedure with the control and phosphorylated peptides. The phospho specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100.
Mediates stress-induced small heat shock protein 27 phosphorylation.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
Contains 1 protein kinase domain.
Phosphorylated on Thr-182; which is the regulatory phosphorylation site and is located on the T-loop/loop 12.
Cytoplasm. Nucleus. Also observed in the nucleus.
Information by UniProt
MAP kinase-activated protein kinase 5 antibody
MAPK-activated protein kinase 5 antibody
Anti-PRAK (phospho S212) antibody images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PRAK (phospho S212) antibody (ab59999)
ab59999 at 1/50 dilution staining PRAK in Human hepatocarcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue.
The primary antibody was peroxidase conjugated to the secondary antibody, followed by AEC staining.
References for Anti-PRAK (phospho S212) antibody (ab59999)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"