Approx 90-100kDa band seen in Jurkat cell lysates. Overnight incubation is recommended with this antibody.
Please note that although the predicted MW of PRAM-1 is 79kDa, Moog-Lutz et al., (J. Biol. Chem, Jun 2001; 276: 22375 - 22381) detected aPRAM-1 migrating with an apparent molecular mass of 97kDa and this is what we observed as well.
FunctionMay be involved in myeloid differentiation. May be involved in integrin signaling in neutrophils. Binds to PtdIns(4)P.
Tissue specificityExpressed in peripheral blood leukocytes and bone marrow. Expressed in monocytes, and to a lesser extent in granulocytes and lymphocytes. Not expressed in non hematopoietic tissues except in lung.
Sequence similaritiesContains 1 SH3 domain.
DomainThe SH3 domain binds to PtdIns(4)P.
Post-translational modificationsMay be phosphorylated on tyrosines.