Overview

  • Product nameAnti-PRAM1 antibody
    See all PRAM1 primary antibodies
  • Description
    Goat polyclonal to PRAM1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    DFCDPLENQPLPLGR

    , corresponding to C terminal amino acids 656-670 of Human PRAM1.

  • Positive control
    • Jurkat cell lysates.
  • General notesPrincipal Names - PRAM-1; PML-RARA target gene encoding an Adaptor Molecule-1 Official Gene Symbol - PRAM-1 GenBank Accession Number – NP_115528 LocusLink ID - 84106


    PRAM-1 is an adaptor protein interacting with proteins involved in an ATRA-signaling pathway

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 0.5mg/ml Tris, pH 7.3
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Primary antibody notesPRAM-1 is an adaptor protein interacting with proteins involved in an ATRA-signaling pathway
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab805 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Can be blocked with Human PRAM1 peptide (ab22785).

Approx 90-100kDa band seen in Jurkat cell lysates. Overnight incubation is recommended with this antibody.

Please note that although the predicted MW of PRAM-1 is 79kDa, Moog-Lutz et al., (J. Biol. Chem, Jun 2001; 276: 22375 - 22381) detected aPRAM-1 migrating with an apparent molecular mass of 97kDa and this is what we observed as well.

Target

  • FunctionMay be involved in myeloid differentiation. May be involved in integrin signaling in neutrophils. Binds to PtdIns(4)P.
  • Tissue specificityExpressed in peripheral blood leukocytes and bone marrow. Expressed in monocytes, and to a lesser extent in granulocytes and lymphocytes. Not expressed in non hematopoietic tissues except in lung.
  • Sequence similaritiesContains 1 SH3 domain.
  • DomainThe SH3 domain binds to PtdIns(4)P.
  • Post-translational
    modifications
    May be phosphorylated on tyrosines.
  • Information by UniProt
  • Database links
  • Alternative names
    • PML RAR antibody
    • PML RARA regulated adaptor molecule 1 antibody
    • PML RARA target gene encoding an Adaptor Molecule 1 antibody
    • PML-RARA-regulated adapter molecule 1 antibody
    • PRAM 1 antibody
    • PRAM antibody
    • PRAM-1 antibody
    • PRAM_HUMAN antibody
    • PRAM1 antibody
    see all

Anti-PRAM1 antibody images

  • staining (2µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 12 hours. Detected by western blot using chemiluminescence

References for Anti-PRAM1 antibody (ab805)

ab805 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"