Overview

  • Product name
  • Description
    Goat polyclonal to PRAM1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    DFCDPLENQPLPLGR

    , corresponding to C terminal amino acids 656-670 of Human PRAM1.

  • Positive control
    • Jurkat cell lysates.
  • General notes
    Principal Names - PRAM-1; PML-RARA target gene encoding an Adaptor Molecule-1 Official Gene Symbol - PRAM-1 GenBank Accession Number – NP_115528 LocusLink ID - 84106


    PRAM-1 is an adaptor protein interacting with proteins involved in an ATRA-signaling pathway

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 0.5mg/ml Tris, pH 7.3
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Primary antibody notes
    PRAM-1 is an adaptor protein interacting with proteins involved in an ATRA-signaling pathway
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab805 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Can be blocked with Human PRAM1 peptide (ab22785).

Approx 90-100kDa band seen in Jurkat cell lysates. Overnight incubation is recommended with this antibody.

Please note that although the predicted MW of PRAM-1 is 79kDa, Moog-Lutz et al., (J. Biol. Chem, Jun 2001; 276: 22375 - 22381) detected aPRAM-1 migrating with an apparent molecular mass of 97kDa and this is what we observed as well.

Target

  • Function
    May be involved in myeloid differentiation. May be involved in integrin signaling in neutrophils. Binds to PtdIns(4)P.
  • Tissue specificity
    Expressed in peripheral blood leukocytes and bone marrow. Expressed in monocytes, and to a lesser extent in granulocytes and lymphocytes. Not expressed in non hematopoietic tissues except in lung.
  • Sequence similarities
    Contains 1 SH3 domain.
  • Domain
    The SH3 domain binds to PtdIns(4)P.
  • Post-translational
    modifications
    May be phosphorylated on tyrosines.
  • Information by UniProt
  • Database links
  • Alternative names
    • PML RAR antibody
    • PML RARA regulated adaptor molecule 1 antibody
    • PML RARA target gene encoding an Adaptor Molecule 1 antibody
    • PML-RARA-regulated adapter molecule 1 antibody
    • PRAM 1 antibody
    • PRAM antibody
    • PRAM-1 antibody
    • PRAM_HUMAN antibody
    • PRAM1 antibody
    see all

Images

  • staining (2µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 12 hours. Detected by western blot using chemiluminescence

References

ab805 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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