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Synthetic peptide within Human PRAMEF6 aa 402-431 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: Q5VXH4
Our Abpromise guarantee covers the use of ab170425 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/500. Predicted molecular weight: 55 kDa.|
|IHC-P||1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|Flow Cyt||1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/10 - 1/50.|
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue labeling PRAMEF6 with ab170425 at 1/10 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.
Immunofluorescent analysis of NCI-H460 cells labeling PRAMEF6 with ab170425 at 1/10 dilution, followed by secondary antibody labeling with Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of NCI-H460 cells labeling PRAMEF6 with ab170425 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ab170425 has not yet been referenced specifically in any publications.