The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
1/50 - 1/100.
FunctionSubunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection.
Tissue specificityWidely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A549 and HeLa) than in normal cell lines (e.g. HEK293).
Post-translational modificationsPhosphorylated by AKT1. Phosphorylation relieves inhibitory function on mTORC1.
Cellular localizationCytoplasm > cytosol. Found in the cytosolic fraction of the brain.
Anti-PRAS40 antibody (ab59438) at 1 µg/ml + Testis (Human) Tissue Lysate - adult normal tissue (ab30257) at 10 µg
Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 57 kDa Observed band size : 57 kDa
Anti-PRAS40 antibody (ab59438)
Immunohistochemical analysis of paraffin embedded human brain tissue stained with ab59438 at 1/50 dilution; in the presence or absence of the immunizing peptide .Immunohistochemical analysis of paraffin embedded human brain tissue stained with ab59438 at 1/50 dilution; in the presence or absence of the immunizing peptide .
References for Anti-PRAS40 antibody (ab59438)
has not yet been referenced specifically in any publications.
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