Overview

  • Product nameAnti-PRAS40 antibody
    See all PRAS40 primary antibodies
  • Description
    Rabbit polyclonal to PRAS40
  • SpecificityPRAS40 Antibody detects endogenous levels of total PRAS40 protein.
  • Tested applicationsWB, IHC, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human PRAS40. Synthetic non-phosphopeptide (Human) from around the phosphorylation site of threonine 246 (LNTPSD)
    Database link: Q96B36

  • Positive control
    • Human brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab59438 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
IHC Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
ELISA 1/5000.
IHC-P 1/50 - 1/100.

Target

  • FunctionSubunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection.
  • Tissue specificityWidely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A549 and HeLa) than in normal cell lines (e.g. HEK293).
  • Post-translational
    modifications
    Phosphorylated by AKT1. Phosphorylation relieves inhibitory function on mTORC1.
  • Cellular localizationCytoplasm > cytosol. Found in the cytosolic fraction of the brain.
  • Information by UniProt
  • Database links
  • Alternative names
    • AKT 1 antibody
    • AKT antibody
    • AKT1 antibody
    • AKT1_HUMAN antibody
    • C AKT antibody
    • MGC9965 antibody
    • MGC99656 antibody
    • Oncogene AKT1 antibody
    • PKB antibody
    • PKB-ALPHA antibody
    • PRKBA antibody
    • Protein Kinase B Alpha antibody
    • Protein kinase B antibody
    • Proto-oncogene c-Akt antibody
    • RAC Alpha antibody
    • RAC antibody
    • RAC PK Alpha antibody
    • RAC Serine/Threonine Protein Kinase antibody
    • RAC-alpha serine/threonine-protein kinase antibody
    • RAC-PK-alpha antibody
    • vAKT Murine Thymoma Viral Oncogene Homolog 1 antibody
    see all

Anti-PRAS40 antibody images

  • Anti-PRAS40 antibody (ab59438) at 1 µg/ml + Testis (Human) Tissue Lysate - adult normal tissue (ab30257) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 57 kDa
    Observed band size : 57 kDa
  • Immunohistochemical analysis of paraffin embedded human brain tissue stained with ab59438 at 1/50 dilution; in the presence or absence of the immunizing peptide .Immunohistochemical analysis of paraffin embedded human brain tissue stained with ab59438 at 1/50 dilution; in the presence or absence of the immunizing peptide .

References for Anti-PRAS40 antibody (ab59438)

ab59438 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"