• Product nameAnti-PRAS40 antibody
    See all PRAS40 primary antibodies
  • Description
    Rabbit polyclonal to PRAS40
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residues 195 and 245 of human PRAS40, (NP_115751.1).

  • Positive control
    • HeLa and 293T whole cell lysates.


Associated products


Our Abpromise guarantee covers the use of ab72321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
IP Use at 2-5 µg/mg of lysate.


  • FunctionSubunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection.
  • Tissue specificityWidely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A549 and HeLa) than in normal cell lines (e.g. HEK293).
  • Post-translational
    Phosphorylated by AKT1. Phosphorylation relieves inhibitory function on mTORC1.
  • Cellular localizationCytoplasm > cytosol. Found in the cytosolic fraction of the brain.
  • Information by UniProt
  • Database links
  • Alternative names
    • 40 kDa proline rich AKT substrate antibody
    • 40 kDa proline-rich AKT substrate antibody
    • AKT1 S1 antibody
    • AKT1 substrate 1 (proline rich) antibody
    • AKT1 substrate 1 antibody
    • AKT1S 1 antibody
    • AKT1S1 antibody
    • AKTS1_HUMAN antibody
    • Lobe antibody
    • MGC2865 antibody
    • PRAS 40 antibody
    • PRAS antibody
    • PRAS40 antibody
    • Proline rich akt substrate antibody
    • Proline rich Akt substrate 40 kDa antibody
    • Proline-rich AKT1 substrate 1 antibody
    see all

Anti-PRAS40 antibody images

  • All lanes : Anti-PRAS40 antibody (ab72321) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Predicted band size : 40 kDa
    Observed band size : 40 kDa
    Additional bands at : 30 kDa,50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • Detection of PRAS40 by Western Blot of Immunprecipitate.
    ab72321 at 1µg/ml for WB, staining PRAS40 in HeLa whole cell lysate subsequent to immunoprecipitation using ab72321 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 10 seconds.

References for Anti-PRAS40 antibody (ab72321)

ab72321 has not yet been referenced specifically in any publications.

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