The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Tissue specificityPredominantly expressed in brain, pituitary gland and thymus in adults. In adult brain, highest expression found in temporal lobe and the putamen, followed by amygdala, caudate nucleus, cerebral cortex, occipital and frontal lobe. A high expression level is also observed in fetal tissues like brain, heart, kidney and thymus.
Sequence similaritiesBelongs to the RIMBP family. Contains 3 fibronectin type-III domains. Contains 3 SH3 domains.
DomainThe SH3 and proline-rich domain is required for the interaction with BZRP and the second SH3 domain mediates binding to a proline-rich motif in RIMS1 and RIMS2.
Cellular localizationCytoplasm. Mitochondrion. Preferentially expressed in the mitochondria in the presence of BZRP.