Overview

  • Product nameAnti-PRC1 antibody
    See all PRC1 primary antibodies
  • Description
    Rabbit polyclonal to PRC1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, between internal sequence amino acids 178-207 of Human PRC1.

  • Positive control
    • MDA-MB231 cell line

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityAmmonium Sulphate Precipitation
  • Purification notesab103096 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab103096 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Predicted molecular weight: 72 kDa.

Target

  • FunctionCross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody.
  • Sequence similaritiesBelongs to the MAP65/ASE1 family.
  • DomainMicrotubule binding occurs via a basic patch in the central spectrin-like domain and requires also the unstructured C-terminal domain.
  • Post-translational
    modifications
    Phosphorylation by CDK1 in early mitosis holds PRC1 in an inactive monomeric state, during the metaphase to anaphase transition, PRC1 is dephosphorylated, promoting interaction with KIF4A, which then translocates PRC1 along mitotic spindles to the plus ends of antiparallel interdigitating microtubules. Dephosphorylation also promotes MT-bundling activity by allowing dimerization.
  • Cellular localizationNucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle pole. Predominantly localized to the nucleus of interphase cells. During mitosis becomes associated with the mitotic spindle poles and localizes with the cell midbody during cytokinesis.
  • Information by UniProt
  • Database links
  • Alternative names
    • Anaphase spindle elongation 1 homolog antibody
    • ASE1 antibody
    • PRC1 antibody
    • PRC1_HUMAN antibody
    • Protein regulating cytokinesis 1 antibody
    • Protein regulator of cytokinesis 1 antibody
    see all

Anti-PRC1 antibody images

  • Anti-PRC1 antibody (ab103096) at 1/50 dilution + MDA-MB231 cell lysate at 35 µg

    Predicted band size : 72 kDa

References for Anti-PRC1 antibody (ab103096)

ab103096 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"