Overview

  • Product nameAnti-PRC1 (phospho T481) antibody [EP1514Y]
    See all PRC1 primary antibodies
  • Description
    Rabbit monoclonal [EP1514Y] to PRC1 (phospho T481)
  • Specificityab62366 detects PRC1 phosphorylated at Theonine 481.
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A phosphopeptide corresponding to residues surrounding Threonine 481 of human PRC1

  • Positive control
    • HeLa cells and HeLa cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberEP1514Y
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab62366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/500. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionCross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody.
    • Sequence similaritiesBelongs to the MAP65/ASE1 family.
    • DomainMicrotubule binding occurs via a basic patch in the central spectrin-like domain and requires also the unstructured C-terminal domain.
    • Post-translational
      modifications
      Phosphorylation by CDK1 in early mitosis holds PRC1 in an inactive monomeric state, during the metaphase to anaphase transition, PRC1 is dephosphorylated, promoting interaction with KIF4A, which then translocates PRC1 along mitotic spindles to the plus ends of antiparallel interdigitating microtubules. Dephosphorylation also promotes MT-bundling activity by allowing dimerization.
    • Cellular localizationNucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle pole. Predominantly localized to the nucleus of interphase cells. During mitosis becomes associated with the mitotic spindle poles and localizes with the cell midbody during cytokinesis.
    • Information by UniProt
    • Database links
    • Alternative names
      • Anaphase spindle elongation 1 homolog antibody
      • ASE1 antibody
      • PRC1 antibody
      • PRC1_HUMAN antibody
      • Protein regulating cytokinesis 1 antibody
      • Protein regulator of cytokinesis 1 antibody
      see all

    Anti-PRC1 (phospho T481) antibody [EP1514Y] images

    • All lanes : Anti-PRC1 (phospho T481) antibody [EP1514Y] (ab62366) at 1/500 dilution

      Lane 1 : HeLa cell lysates (untreated)
      Lane 2 : HeLa cell lysates treated with aphidicolin + nocodazole

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP labeled, at 1/2000 dilution

      Predicted band size : 72 kDa
      Observed band size : 72 kDa
    • ab62366 at 1/100 dilution staining PRC1 in HeLa cells by Immunocytochemistry.

    References for Anti-PRC1 (phospho T481) antibody [EP1514Y] (ab62366)

    This product has been referenced in:
    • Cundell MJ  et al. A PP2A-B55 recognition signal controls substrate dephosphorylation kinetics during mitotic exit. J Cell Biol 214:539-54 (2016). Read more (PubMed: 27551054) »

    See 1 Publication for this product

    Product Wall

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"