Overview

  • Product name
  • Description
    Mouse polyclonal to PRCP
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human PRCP protein (NP_005031)

  • Positive control
    • Human kidney tissue lysate and transfected 293T cell line lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab72942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/500 - 1/1000. Detects a band of approximately 37 kDa (tissue lysate) and 57 kDa (predicted molecular weight: 57 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Cleaves C-terminal amino acids linked to proline in peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH.
    • Tissue specificity
      Highest levels in placenta, lung and liver. Also present in heart, brain, pancreas and kidney.
    • Sequence similarities
      Belongs to the peptidase S28 family.
    • Cellular localization
      Lysosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • Angiotensinase C antibody
      • HUMPCP antibody
      • Lysosomal carboxypeptidase C antibody
      • Lysosomal Pro X carboxypeptidase antibody
      • Lysosomal Pro-X carboxypeptidase antibody
      • MGC2202 antibody
      • PCP antibody
      • PCP_HUMAN antibody
      • PRCP antibody
      • Proline carboxypeptidase antibody
      • Prolylcarboxypeptidase (angiotensinase C) antibody
      • Prolylcarboxypeptidase antibody
      • prolylcarboxypeptidase isoform 1 preproprotein antibody
      see all

    Images

    • Anti-PRCP antibody (ab72942) at 1/500 dilution + human kidney tissue lysate at 25 µg

      Secondary
      Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

      Predicted band size : 57 kDa
      Observed band size : 57 kDa
      Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.
    • All lanes : Anti-PRCP antibody (ab72942) at 1/500 dilution

      Lane 1 : Transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

      Predicted band size : 57 kDa
      Observed band size : 57 kDa

    References

    This product has been referenced in:
    • Duan L  et al. Prolylcarboxypeptidase Regulates Proliferation, Autophagy, and Resistance to 4-Hydroxytamoxifen-induced Cytotoxicity in Estrogen Receptor-positive Breast Cancer Cells. J Biol Chem 286:2864-76 (2011). WB ; Human . Read more (PubMed: 21087932) »

    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab72942.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up