Anti-PRDM10 antibody [48AT1224.90.46] (ab3787)

Overview

  • Product name
    Anti-PRDM10 antibody [48AT1224.90.46]
    See all PRDM10 primary antibodies
  • Description
    Mouse monoclonal [48AT1224.90.46] to PRDM10
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human PRDM10 aa 50-79 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • Positive control
    • 293 cell lysate.
  • General notes


    Similar to acetylation and phosphorylation, histone methylation at the N-terminal tail has emerged as an important role in regulating chromatin dynamics and gene activity. Histone methylation occurs on arginine and lysine residues and is catalyzed by two families of proteins, the protein arginine methyltransferase family and the SET-domain-containing methyltransferase family. Five members have been identified in the arginine methyltransferase family. About 27 are grouped into the SET-domain family, and another 17 make up the PR domain family that is related to the SET domain family. The retinoblastoma protein-interacting zinc finger gene RIZ 1 is a tumor suppressor gene and a FOUNDING member of the PR domain family. RIZ 1 inactivation is commonly found in many types of human cancers and occurs through loss of mRNA expression, frame shift mutation, chromosomal deletion, and missense mutation. RIZ 1 is also a tumor susceptibility gene in mice. The loss of RIZ 1 mRNA in human cancers was shown to associate with DNA methylation of its promoter CpG island. Methylation of the RIZ 1 promoter strongly correlated with lost or decreased RIZ 1 mRNA expression in breast, liver, colon, and lung cancer cell lines as well as in liver cancer tissues.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    PBS with 0.1% sodium azide
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    This antibody is purified through a protein G column and eluted out with both high and low pH buffers. It is neutralized immediately after elution, followed by dialysis against PBS.
  • Primary antibody notes
    Similar to acetylation and phosphorylation, histone methylation at the N-terminal tail has emerged as an important role in regulating chromatin dynamics and gene activity. Histone methylation occurs on arginine and lysine residues and is catalyzed by two families of proteins, the protein arginine methyltransferase family and the SET-domain-containing methyltransferase family. Five members have been identified in the arginine methyltransferase family. About 27 are grouped into the SET-domain family, and another 17 make up the PR domain family that is related to the SET domain family. The retinoblastoma protein-interacting zinc finger gene RIZ 1 is a tumor suppressor gene and a FOUNDING member of the PR domain family. RIZ 1 inactivation is commonly found in many types of human cancers and occurs through loss of mRNA expression, frame shift mutation, chromosomal deletion, and missense mutation. RIZ 1 is also a tumor susceptibility gene in mice. The loss of RIZ 1 mRNA in human cancers was shown to associate with DNA methylation of its promoter CpG island. Methylation of the RIZ 1 promoter strongly correlated with lost or decreased RIZ 1 mRNA expression in breast, liver, colon, and lung cancer cell lines as well as in liver cancer tissues.
  • Clonality
    Monoclonal
  • Clone number
    48AT1224.90.46
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 120.7 kDa.
ELISA 1/1000.

Target

  • Function
    May be involved in transcriptional regulation.
  • Sequence similarities
    Contains 10 C2H2-type zinc fingers.
    Contains 1 SET domain.
  • Domain
    The SET domain is degenerated, suggesting that it has lost mehtyltransferase activity.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA1231 antibody
    • MGC131802 antibody
    • PFM7 antibody
    • PR domain containing 10 antibody
    • PR domain containing protein 10 antibody
    • PR domain family member 7 antibody
    • PR domain zinc finger protein 10 antibody
    • PR domain-containing protein 10 antibody
    • PRD10_HUMAN antibody
    • PRDM zinc finger transcription factor antibody
    • prdm10 antibody
    • TRIS antibody
    • Tristanin antibody
    see all

Anti-PRDM10 antibody [48AT1224.90.46] images

  • Anti-PRDM10 antibody [48AT1224.90.46] (ab3787) + Extracts from 293 cells

    Predicted band size : 120.7 kDa

References for Anti-PRDM10 antibody [48AT1224.90.46] (ab3787)

ab3787 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HCT116 cells)
Loading amount
50 µg
Specification
HCT116 cells
Gel Running Conditions
Reduced Denaturing (6-15% gradient gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Sep 23 2011

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