Anti-PRDM10 antibody [48AT1224.90.46] (ab3787)

Overview

  • Product nameAnti-PRDM10 antibody [48AT1224.90.46]
    See all PRDM10 primary antibodies
  • Description
    Mouse monoclonal [48AT1224.90.46] to PRDM10
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human PRDM10 aa 50-79 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • Positive control
    • 293 cell lysate.
  • General notes


    Similar to acetylation and phosphorylation, histone methylation at the N-terminal tail has emerged as an important role in regulating chromatin dynamics and gene activity. Histone methylation occurs on arginine and lysine residues and is catalyzed by two families of proteins, the protein arginine methyltransferase family and the SET-domain-containing methyltransferase family. Five members have been identified in the arginine methyltransferase family. About 27 are grouped into the SET-domain family, and another 17 make up the PR domain family that is related to the SET domain family. The retinoblastoma protein-interacting zinc finger gene RIZ 1 is a tumor suppressor gene and a FOUNDING member of the PR domain family. RIZ 1 inactivation is commonly found in many types of human cancers and occurs through loss of mRNA expression, frame shift mutation, chromosomal deletion, and missense mutation. RIZ 1 is also a tumor susceptibility gene in mice. The loss of RIZ 1 mRNA in human cancers was shown to associate with DNA methylation of its promoter CpG island. Methylation of the RIZ 1 promoter strongly correlated with lost or decreased RIZ 1 mRNA expression in breast, liver, colon, and lung cancer cell lines as well as in liver cancer tissues.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPBS with 0.1% sodium azide
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesThis antibody is purified through a protein G column and eluted out with both high and low pH buffers. It is neutralized immediately after elution, followed by dialysis against PBS.
  • Primary antibody notesSimilar to acetylation and phosphorylation, histone methylation at the N-terminal tail has emerged as an important role in regulating chromatin dynamics and gene activity. Histone methylation occurs on arginine and lysine residues and is catalyzed by two families of proteins, the protein arginine methyltransferase family and the SET-domain-containing methyltransferase family. Five members have been identified in the arginine methyltransferase family. About 27 are grouped into the SET-domain family, and another 17 make up the PR domain family that is related to the SET domain family. The retinoblastoma protein-interacting zinc finger gene RIZ 1 is a tumor suppressor gene and a FOUNDING member of the PR domain family. RIZ 1 inactivation is commonly found in many types of human cancers and occurs through loss of mRNA expression, frame shift mutation, chromosomal deletion, and missense mutation. RIZ 1 is also a tumor susceptibility gene in mice. The loss of RIZ 1 mRNA in human cancers was shown to associate with DNA methylation of its promoter CpG island. Methylation of the RIZ 1 promoter strongly correlated with lost or decreased RIZ 1 mRNA expression in breast, liver, colon, and lung cancer cell lines as well as in liver cancer tissues.
  • ClonalityMonoclonal
  • Clone number48AT1224.90.46
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 120.7 kDa.
ELISA 1/1000.

Target

  • FunctionMay be involved in transcriptional regulation.
  • Sequence similaritiesContains 10 C2H2-type zinc fingers.
    Contains 1 SET domain.
  • DomainThe SET domain is degenerated, suggesting that it has lost mehtyltransferase activity.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA1231 antibody
    • MGC131802 antibody
    • PFM7 antibody
    • PR domain containing 10 antibody
    • PR domain containing protein 10 antibody
    • PR domain family member 7 antibody
    • PR domain zinc finger protein 10 antibody
    • PR domain-containing protein 10 antibody
    • PRD10_HUMAN antibody
    • PRDM zinc finger transcription factor antibody
    • prdm10 antibody
    • TRIS antibody
    • Tristanin antibody
    see all

Anti-PRDM10 antibody [48AT1224.90.46] images

  • Anti-PRDM10 antibody [48AT1224.90.46] (ab3787) + Extracts from 293 cells

    Predicted band size : 120.7 kDa

References for Anti-PRDM10 antibody [48AT1224.90.46] (ab3787)

ab3787 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HCT116 cells)
Loading amount 50 µg
Specification HCT116 cells
Gel Running Conditions Reduced Denaturing (6-15% gradient gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Sep 23 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"