Overview

  • Product nameAnti-PRDM10 antibody
    See all PRDM10 primary antibodies
  • Description
    Rabbit polyclonal to PRDM10
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 1066-1115 (VATPVTTGQV KAVTSGHYVL SESQSELEEK QTSALSGGVQ VEPPAHSDSL) of Human PRDM10 (Uniprot = Q9NQV6).

  • Positive control
    • THP1 cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab101956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 130 kDa. Observed a 150 kDa band which is correct for full length PRDM10 isoforms 3, 4 and 6 with some slight post-translational modifications. Degradation products observed at 45-60 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionMay be involved in transcriptional regulation.
  • Sequence similaritiesContains 10 C2H2-type zinc fingers.
    Contains 1 SET domain.
  • DomainThe SET domain is degenerated, suggesting that it has lost mehtyltransferase activity.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA1231 antibody
    • MGC131802 antibody
    • PFM7 antibody
    • PR domain containing 10 antibody
    • PR domain containing protein 10 antibody
    • PR domain family member 7 antibody
    • PR domain zinc finger protein 10 antibody
    • PR domain-containing protein 10 antibody
    • PRD10_HUMAN antibody
    • PRDM zinc finger transcription factor antibody
    • prdm10 antibody
    • TRIS antibody
    • Tristanin antibody
    see all

Anti-PRDM10 antibody images

  • Anti-PRDM10 antibody (ab101956) at 1 µg/ml + THP1 cell lysate at 10 µg
    Developed using the ECL technique

    Predicted band size : 130 kDa

References for Anti-PRDM10 antibody (ab101956)

ab101956 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (HCT116 cells)
Loading amount 50 µg
Specification HCT116 cells
Gel Running Conditions Reduced Denaturing (6-15% gradient gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Oct 03 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"