The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 130 kDa. Observed a 150 kDa band which is correct for full length PRDM10 isoforms 3, 4 and 6 with some slight post-translational modifications. Degradation products observed at 45-60 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionMay be involved in transcriptional regulation.
Sequence similaritiesContains 10 C2H2-type zinc fingers. Contains 1 SET domain.
DomainThe SET domain is degenerated, suggesting that it has lost mehtyltransferase activity.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.