Overview

  • Product nameAnti-PRDM13 antibody
    See all PRDM13 primary antibodies
  • Description
    Rabbit polyclonal to PRDM13
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Cat, Dog, Pig
  • Immunogen

    A region within synthetic peptide: VKSRHPGQSL LAKAGDGPGA EPGYPPEPGD PKSDDSDVDV CFTDDQSDPE, corresponding to C terminal amino acids 649-698 of Human PRDM13

  • Positive control
    • Jurkat cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab66665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 75 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

Target

Anti-PRDM13 antibody images

  • All lanes : Anti-PRDM13 antibody (ab66665) at 1.25 µg/ml

    Lane 1 : Marker
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 75 kDa
    Observed band size : 68 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 20 kDa,36 kDa. We are unsure as to the identity of these extra bands.

References for Anti-PRDM13 antibody (ab66665)

ab66665 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Sample Mouse Cell (Two Cell Embryo)
Specification Two Cell Embryo
Permeabilization Yes - 0.5% Triton for 10 min
Fixative Paraformaldehyde
Username

Dr. jesse mager

Verified customer

Submitted Jan 08 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"