Overview

  • Product nameAnti-PRDM14 antibody
    See all PRDM14 primary antibodies
  • Description
    Rabbit polyclonal to PRDM14
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Cow, Cat, Dog, Pig
  • Immunogen

    A region within a synthetic peptide: ASLHHAISGL LVPPDSSGSD SLPQTLDKDS LQLPEGLCLM QTVFGEVPHF, corresponding to internal sequence amino acids 217-266 of Human PRDM14

  • Positive control
    • Jurkat cell lysate for Western blot, Human lung tissue for Immunohistochemistry (paraffin embedded).

Properties

Applications

Our Abpromise guarantee covers the use of ab28638 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/312500.
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 64 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

Target

Anti-PRDM14 antibody images

  • Anti-PRDM14 antibody (ab28638) at 1.25 µg/ml + Jurkat cell lysate 10ug.

    Secondary
    HRP conjugated anti-Rabbit IgG 1: 50,000 - 100,000

    Predicted band size : 64 kDa
    Observed band size : 62 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 41 kDa. We are unsure as to the identity of these extra bands.
  • ab28638 staining PRDM14 in Human lung by Immunohistochemistry (FFPE-sections) at 4-8ug/ml. Positive label Alveolar cells (arrows).

References for Anti-PRDM14 antibody (ab28638)

This product has been referenced in:

See all 2 Publications for this product

Product Wall

Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (293T-FLAG-hPRDM14)
Loading amount 15 µg
Specification 293T-FLAG-hPRDM14
Treatment transcient transfected 293T with FLAG-hPRDM14
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Dec 19 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"