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A 17 amino acid peptide from near the carboxy terminus of Human PRDM16 (CAH71530).
Our Abpromise guarantee covers the use of ab106410 in the following tested applications.
|ICC/IF||Use a concentration of 20 µg/ml.|
|IHC-P||Use a concentration of 2.5 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 140 kDa.|
ab106410 staining RAP1 in Human Brown adipocytes from supraclavicular fat tissue by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 10% serum, 1% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (2 ug/ml in 10% serum, 1% BSA, 0.3% Triton X100, PBS) for 16 hours at 4°C. A Cy3®-conjugated Donkey F(ab')2 anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
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