Anti-PRDM16 antibody (ab106410)
Key features and details
- Rabbit polyclonal to PRDM16
- Suitable for: ICC, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PRDM16 antibody
See all PRDM16 primary antibodies -
Description
Rabbit polyclonal to PRDM16 -
Host species
Rabbit -
Specificity
At least four isoforms of PRDM16 are known to exist; ab106410 will recognize all four. -
Tested applications
Suitable for: ICC, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human PRDM16 (C terminal). The immunogen is located within amino acids 1120 - 1170 of PRDM16.
Database link: Q9HAZ2 -
Positive control
- WB: K562 cell lysate IHC-P: Human brain tissue ICC: Human brain cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106410 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use a concentration of 20 µg/ml.
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IHC-P | (3) |
Use a concentration of 2 µg/ml.
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WB | (3) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 140 kDa.
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Notes |
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ICC
Use a concentration of 20 µg/ml. |
IHC-P
Use a concentration of 2 µg/ml. |
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 140 kDa. |
Target
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Function
Binds DNA and functions as a transcriptional regulator. Functions in the differentiation of brown adipose tissue (BAT) which is specialized in dissipating chemical energy in the form of heat in response to cold or excess feeding while white adipose tissue (WAT) is specialized in the storage of excess energy and the control of systemic metabolism. Together with CEBPB, regulates the differentiation of myoblastic precursors into brown adipose cells. Functions also as a repressor of TGF-beta signaling. Isoform 4 may regulate granulocytes differentiation. -
Tissue specificity
Expressed in uterus and kidney. -
Involvement in disease
Note=A chromosomal aberration involving PRDM16 is found in myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). Reciprocal translocation t(1;3)(p36;q21). Isoform 4 is specifically expressed in adult T-cell leukemia. -
Sequence similarities
Contains 10 C2H2-type zinc fingers.
Contains 1 SET domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 63976 Human
- Omim: 605557 Human
- SwissProt: Q9HAZ2 Human
- Unigene: 99500 Human
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Alternative names
- CMD1LL antibody
- KIAA1675 antibody
- LVNC8 antibody
see all
Images
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Anti-PRDM16 antibody (ab106410) at 1 µg/ml + K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Predicted band size: 140 kDa -
Paraffin-embedded human brain tissue stained for PRDM16 using ab106410 at 2 µg/ml in immunohistochemical analysis.
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ab106410 staining RAP1 in Human Brown adipocytes from supraclavicular fat tissue by ICC (Immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 10% serum, 1% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (2 ug/ml in 10% serum, 1% BSA, 0.3% Triton X100, PBS) for 16 hours at 4°C. A Cy3®-conjugated Donkey F(ab')2 anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
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Immunocytochemical analysis of PRDM16 in Human Brain cells using ab106410 at 20 ug/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (69)
ab106410 has been referenced in 69 publications.
- He X et al. FNDC5/irisin facilitates muscle-adipose-bone connectivity through ubiquitination-dependent activation of runt-related transcriptional factors RUNX1/2. J Biol Chem 298:101679 (2022). PubMed: 35124008
- Yang F et al. The gut microbiota mediates protective immunity against tuberculosis via modulation of lncRNA. Gut Microbes 14:2029997 (2022). PubMed: 35343370
- de Melo DG et al. Strength training alters the tissue fatty acids profile and slightly improves the thermogenic pathway in the adipose tissue of obese mice. Sci Rep 12:6913 (2022). PubMed: 35484170
- Turner AW et al. Single-nucleus chromatin accessibility profiling highlights regulatory mechanisms of coronary artery disease risk. Nat Genet 54:804-816 (2022). PubMed: 35590109
- Guo F et al. DEPP Deficiency Contributes to Browning of White Adipose Tissue. Int J Mol Sci 23:N/A (2022). PubMed: 35743009