• Product name
  • Description
    Rabbit polyclonal to PRDM16
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1250 to the C-terminus of Human PRDM16 .

    (Peptide available as ab95842.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; HEK293.



Our Abpromise guarantee covers the use of ab85874 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 140 kDa).


  • Function
    Binds DNA and functions as a transcriptional regulator. Functions in the differentiation of brown adipose tissue (BAT) which is specialized in dissipating chemical energy in the form of heat in response to cold or excess feeding while white adipose tissue (WAT) is specialized in the storage of excess energy and the control of systemic metabolism. Together with CEBPB, regulates the differentiation of myoblastic precursors into brown adipose cells. Functions also as a repressor of TGF-beta signaling. Isoform 4 may regulate granulocytes differentiation.
  • Tissue specificity
    Expressed in uterus and kidney.
  • Involvement in disease
    Note=A chromosomal aberration involving PRDM16 is found in myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). Reciprocal translocation t(1;3)(p36;q21). Isoform 4 is specifically expressed in adult T-cell leukemia.
  • Sequence similarities
    Contains 10 C2H2-type zinc fingers.
    Contains 1 SET domain.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • CMD1LL antibody
    • KIAA1675 antibody
    • LVNC8 antibody
    • MDS1/EVI1 like gene 1 antibody
    • MDS1/EVI1-like gene 1 antibody
    • MEL1 antibody
    • PFM 13 antibody
    • PFM13 antibody
    • PR domain containing 16 antibody
    • PR domain containing protein 16 antibody
    • PR domain zinc finger protein 16 antibody
    • PR domain-containing protein 16 antibody
    • PRD16_HUMAN antibody
    • Prdm16 antibody
    • Transcription factor MEL 1 antibody
    • Transcription factor MEL1 antibody
    see all


  • All lanes : Anti-PRDM16 antibody (ab85874) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg/ml per lane.

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 140 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)

    Exposure time : 12 minutes


ab85874 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Merci d'avoir pris le temps de remplir notre questionnaire et de nous avoir contactés.

Notre politique est d'offrir tout d'abord la meilleure assistance technique. Si nous concluons que le produit que vous avez reçu ne fonctionn...

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Je suis désolée d'apprendre que ab85874 ne vous donne pas de bons résultats en Western blot.

Comme discuté au téléphne, veuillez trouver ci-joint un questionnaire qui nous permet de regrouper le plus...

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