Anti-PRDM1/Blimp1 antibody [3H2-E8] (Biotin) (ab81961)

Overview

  • Product nameAnti-PRDM1/Blimp1 antibody [3H2-E8] (Biotin)
    See all PRDM1/Blimp1 primary antibodies
  • Description
    Mouse monoclonal [3H2-E8] to PRDM1/Blimp1 (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: WB, ICC, ICC/IF, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Protein fragment, corresponding to amino acids 199-409 of Mouse PRDM1/Blimp1

  • Positive control
    • P3X (murine plasmocytoma) cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab81961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 95 kDa.
ICC 1/50 - 1/100.
ICC/IF 1/200.
IHC-P Use at an assay dependent dilution.
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionTranscriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene (PubMed:1851123). Drives the maturation of B-lymphocytes into Ig secreting cells (PubMed:12626569).
    • Sequence similaritiesBelongs to the class V-like SAM-binding methyltransferase superfamily.
      Contains 4 C2H2-type zinc fingers.
      Contains 1 SET domain.
    • Post-translational
      modifications
      Sumoylation at Lys-816 by PIAS1 augments transcriptional repressor activity, and is critical for plasma cell differentiation.
      Ubiquitinated by the SCF(FBXO11) complex, leading to its degradation by the proteasome.
    • Cellular localizationNucleus. Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • B Lymphocyte Induced Maturation Protein 1 antibody
      • Beta interferon gene positive regulatory domain I binding factor antibody
      • Beta-interferon gene positive regulatory domain I-binding factor antibody
      • BLIMP-1 antibody
      • BLIMP1 antibody
      • Positive Regulatory Domain I Binding Factor 1 antibody
      • Positive regulatory domain I-binding factor 1 antibody
      • PR Domain Containing 1 antibody
      • PR domain containing 1 with ZNF domain antibody
      • PR domain containing 1 with ZNF domain isoform 2 antibody
      • PR domain containing protein 1 antibody
      • PR domain zinc finger protein 1 antibody
      • PR domain-containing protein 1 antibody
      • PRDI BF1 antibody
      • PRDI binding factor 1 antibody
      • PRDI-BF1 antibody
      • PRDI-binding factor 1 antibody
      • PRDM 1 antibody
      • Prdm1 antibody
      • PRDM1_HUMAN antibody
      see all

    References for Anti-PRDM1/Blimp1 antibody [3H2-E8] (Biotin) (ab81961)

    ab81961 has not yet been referenced specifically in any publications.

    Product Wall

    Application Flow Cytometry
    Fixation Formaldehyde
    Permeabilization Yes - Triton X100
    Sample Mouse Cell (spleen cells)
    Specification spleen cells
    Gating Strategy CD8 T cells
    Preparation Cell harvesting/tissue preparation method: single cell suspension
    Sample buffer: PBS / FCS
    Username

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    Verified customer

    Submitted Jun 19 2013

    Thanks for your email. ab4587 does not have any required MSDS information, so a link will not be present on this datasheet. For example, ab7254 does have a link to MSDS information. I hope this helps. Please contact me again if you have any further que...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"