Anti-PRDM1/Blimp1 antibody (ab119401)
Key features and details
- Rabbit polyclonal to PRDM1/Blimp1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PRDM1/Blimp1 antibody
See all PRDM1/Blimp1 primary antibodies -
Description
Rabbit polyclonal to PRDM1/Blimp1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide, corresponding to a sequence around amino acids 124-128 (
DTVPK
) of Human PRDM1/Blimp1 (NP_001189.2 ). -
Positive control
- Jurkat an Raji cell extracts
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
(without Mg2+ and Ca2+) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab119401 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/1000. Detects a band of approximately 95-100 kDa (predicted molecular weight: 93 kDa).
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 95-100 kDa (predicted molecular weight: 93 kDa). |
Target
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Function
Transcriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene (PubMed:1851123). Drives the maturation of B-lymphocytes into Ig secreting cells (PubMed:12626569). -
Sequence similarities
Belongs to the class V-like SAM-binding methyltransferase superfamily.
Contains 4 C2H2-type zinc fingers.
Contains 1 SET domain. -
Post-translational
modificationsSumoylation at Lys-816 by PIAS1 augments transcriptional repressor activity, and is critical for plasma cell differentiation.
Ubiquitinated by the SCF(FBXO11) complex, leading to its degradation by the proteasome. -
Cellular localization
Nucleus. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 639 Human
- Entrez Gene: 12142 Mouse
- Entrez Gene: 309871 Rat
- Omim: 603423 Human
- SwissProt: O75626 Human
- SwissProt: Q60636 Mouse
- Unigene: 436023 Human
- Unigene: 4800 Mouse
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Alternative names
- B Lymphocyte Induced Maturation Protein 1 antibody
- Beta interferon gene positive regulatory domain I binding factor antibody
- Beta-interferon gene positive regulatory domain I-binding factor antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab119401 has been referenced in 3 publications.
- Zeng F et al. KPNA2 interaction with CBX8 contributes to the development and progression of bladder cancer by mediating the PRDM1/c-FOS pathway. J Transl Med 19:112 (2021). PubMed: 33731128
- Yang J et al. T-cell-derived extracellular vesicles regulate B-cell IgG production via pyruvate kinase muscle isozyme 2. FASEB J 33:12780-12799 (2019). PubMed: 31480861
- Chen JL et al. Suppression of SIK1 by miR-141 in human ovarian cancer cell lines and tissues. Int J Mol Med 37:1601-10 (2016). PubMed: 27081781