The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 95-100 kDa (predicted molecular weight: 93 kDa).
FunctionTranscriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene (PubMed:1851123). Drives the maturation of B-lymphocytes into Ig secreting cells (PubMed:12626569).
Sequence similaritiesBelongs to the class V-like SAM-binding methyltransferase superfamily. Contains 4 C2H2-type zinc fingers. Contains 1 SET domain.
Post-translational modificationsSumoylation at Lys-816 by PIAS1 augments transcriptional repressor activity, and is critical for plasma cell differentiation. Ubiquitinated by the SCF(FBXO11) complex, leading to its degradation by the proteasome.