Anti-PRDM1/Blimp1 antibody - ChIP Grade (ab13700)

Overview

  • Product nameAnti-PRDM1/Blimp1 antibody - ChIP Grade
    See all PRDM1/Blimp1 primary antibodies
  • Description
    Goat polyclonal to PRDM1/Blimp1 - ChIP Grade
  • Tested applicationsSuitable for: Flow Cyt, ChIP, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide: KVKQETVEPMDP, corresponding to C terminal amino acids 778-789 of Human PRDM1/Blimp1.

  • Positive control
    • Human Lymph Node lysate
  • General notesGenBank Accession Number - NP_001189

Properties

Applications

Our Abpromise guarantee covers the use of ab13700 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. ab37373-Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.
ChIP Use at an assay dependent concentration. PubMed: 17264218
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 91 kDa).

Target

  • FunctionTranscriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene (PubMed:1851123). Drives the maturation of B-lymphocytes into Ig secreting cells (PubMed:12626569).
  • Sequence similaritiesBelongs to the class V-like SAM-binding methyltransferase superfamily.
    Contains 4 C2H2-type zinc fingers.
    Contains 1 SET domain.
  • Post-translational
    modifications
    Sumoylation at Lys-816 by PIAS1 augments transcriptional repressor activity, and is critical for plasma cell differentiation.
    Ubiquitinated by the SCF(FBXO11) complex, leading to its degradation by the proteasome.
  • Cellular localizationNucleus. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • B Lymphocyte Induced Maturation Protein 1 antibody
    • Beta interferon gene positive regulatory domain I binding factor antibody
    • Beta-interferon gene positive regulatory domain I-binding factor antibody
    • BLIMP-1 antibody
    • BLIMP1 antibody
    • Positive Regulatory Domain I Binding Factor 1 antibody
    • Positive regulatory domain I-binding factor 1 antibody
    • PR Domain Containing 1 antibody
    • PR domain containing 1 with ZNF domain antibody
    • PR domain containing 1 with ZNF domain isoform 2 antibody
    • PR domain containing protein 1 antibody
    • PR domain zinc finger protein 1 antibody
    • PR domain-containing protein 1 antibody
    • PRDI BF1 antibody
    • PRDI binding factor 1 antibody
    • PRDI-BF1 antibody
    • PRDI-binding factor 1 antibody
    • PRDM 1 antibody
    • Prdm1 antibody
    • PRDM1_HUMAN antibody
    see all

Anti-PRDM1/Blimp1 antibody - ChIP Grade images



  • Predicted band size : 91 kDa

    Ab13700 staining (1µg/ml) of human Lymph Node lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence.

  • Anti-PRDM1/Blimp1 antibody - ChIP Grade (ab13700) at 1 µg/ml (for 1 hour) + Human brain (cerebellum) lysate in RIPA buffer at 35 µg

    Predicted band size : 91 kDa
  • Human RPMI8226 cells stained with ab13700 at 5 µg/ml, incubated for 30 minutes at 4°C.  In parallel, cells were stained with goat IgG at an equivalent concentration to that of PRDM1. The secondary antibody was a FITC-conjugated rabbit anti-goat IgG F(ab)2.  PRDMI expression was analysed by flow cytometry.

    See Abreview

References for Anti-PRDM1/Blimp1 antibody - ChIP Grade (ab13700)

This product has been referenced in:
  • Yan J  et al. BLIMP1 regulates cell growth through repression of p53 transcription. Proc Natl Acad Sci U S A 104:1841-6 (2007). ChIP ; Human . Read more (PubMed: 17264218) »
  • Parekh S  et al. BCL6 programs lymphoma cells for survival and differentiation through distinct biochemical mechanisms. Blood 110:2067-74 (2007). Read more (PubMed: 17545502) »

See all 2 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Testis, embryonic day 15.5)
Permeabilization Yes - 0.1% Triton X-100 in PBS
Specification Testis, embryonic day 15.5
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative Paraformaldehyde
Username

Mr. Bryan Niedenberger

Verified customer

Submitted Apr 28 2016

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Sample Pig Cell (Gonad day 25 fetus)
Specification Gonad day 25 fetus
Permeabilization Yes - citrate antigen retrival
Fixative Paraformaldehyde
Username

Dr. Ramiro Alberio

Verified customer

Submitted Jul 23 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (RPMI8226 cell line)
Specification RPMI8226 cell line
Preparation Cell harvesting/tissue preparation method: The cells were harvested and stained with 7AAD for 20 minutes on ice in the dark before the cells were fixed and permeabilised using the BD Cytofix/Cytoperm kit. Intracellular staining for PRDM1 was performed in Perm/wash in the presence of 10% human AB serum for 30 minutes on ice in the dark. In parallel, cells were stained with goat IgG at an equivalent concentration to that of PRDM1. Following three washes in Perm/wash the cells were stained with FITC-conjugated rabbit anti-goat IgG for 30 min on ice in the dark. Following three washes PRDMI expression was analysed by flow cytometry.
Sample buffer: BD Cytofix/Cytoperm
Fixation Formaldehyde
Permeabilization Yes - BD Cytofix/Cytoperm Kit
Gating Strategy All live RPMI8226 cells were included in the analysis. Dead cells were excluded in FL-3 using 7AAD.
Username

Dr. Angelica Cazaly

Verified customer

Submitted Dec 03 2007

Thank you for your enquiry. We do not have any data that the antibody shows the 2 isoforms on western blots of myeloma cell lysates. We only have the data on the lymphoma specimen. As long as the amino acids 778-789 (KVKQETVEPMDP) are present in b...

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I have received a nice reply from the supplier of the ICC image. He states "We simply used a very standard protocol. Fixed for 20 mins at 4C in 4%PFA, blocked in 10% donkey serum for about 60 mins at room temp, then added primary overnight at 4C, t...

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Thank you for your enquiry. I am sorry to hear that you have been having difficulties with ab13700. The ICC image on the datasheet was submitted by a customer who wishes to remain anonymous. I have contacted him directly requesting further informat...

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Thank you for your reply and for sending us further information about your customer's Western blot protocol. Looking through the original completed Questionnaire and the forwarded extra-information you have just sent to us, I would like to make the...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"