The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.
Use at an assay dependent concentration. PubMed: 17264218
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 91 kDa).
Transcriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene (PubMed:1851123). Drives the maturation of B-lymphocytes into Ig secreting cells (PubMed:12626569).
Belongs to the class V-like SAM-binding methyltransferase superfamily. Contains 4 C2H2-type zinc fingers. Contains 1 SET domain.
Sumoylation at Lys-816 by PIAS1 augments transcriptional repressor activity, and is critical for plasma cell differentiation. Ubiquitinated by the SCF(FBXO11) complex, leading to its degradation by the proteasome.
PR domain containing 1 with ZNF domain isoform 2 antibody
PR domain containing protein 1 antibody
PR domain zinc finger protein 1 antibody
PR domain-containing protein 1 antibody
PRDI BF1 antibody
PRDI binding factor 1 antibody
PRDI-binding factor 1 antibody
PRDM 1 antibody
Western blot - PRDM1/Blimp1 antibody (ab13700)
Ab13700 staining (1µg/ml) of human Lymph Node lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Western blot - Anti-PRDM1/Blimp1 antibody (ab13700)
Anti-PRDM1/Blimp1 antibody - ChIP Grade (ab13700) at 1 µg/ml (for 1 hour) + Human brain (cerebellum) lysate in RIPA buffer at 35 µg
Predicted band size: 91 kDa
Flow Cytometry - PRDM1/Blimp1 antibody (ab13700)This image is courtesy of an Abreview submitted by Dr Angelica Cazaly
Human RPMI8226 cells stained with ab13700 at 5 µg/ml, incubated for 30 minutes at 4°C. In parallel, cells were stained with goat IgG at an equivalent concentration to that of PRDM1. The secondary antibody was a FITC-conjugated rabbit anti-goat IgG F(ab)2. PRDMI expression was analysed by flow cytometry.