Customer reviews and FAQs

Overview

  • Product name
    Anti-PRDM5 antibody
    See all PRDM5 primary antibodies
  • Description
    Rabbit polyclonal to PRDM5
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a portion of amino acids 100-200 of human PRDM5 protein

  • Positive control
    • Human colon tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab79016 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 73 kDa.
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function
    Sequence-specific DNA-binding transcription factor. Represses transcription at least in part by recruitment of the histone methyltransferase EHMT2/G9A and histone deacetylases such as HDAC1. Regulates hematopoiesis-associated protein-coding and microRNA (miRNA) genes.
  • Sequence similarities
    Contains 16 C2H2-type zinc fingers.
    Contains 1 SET domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
    • Alternative names
      • BCS2 antibody
      • PFM 2 antibody
      • PFM2 antibody
      • PR domain containing 5 antibody
      • PR domain containing protein 5 antibody
      • PR domain zinc finger protein 5 antibody
      • PR domain-containing protein 5 antibody
      • PRDM 5 antibody
      • PRDM5 antibody
      • PRDM5 protein antibody
      • PRDM5_HUMAN antibody
      see all

    Anti-PRDM5 antibody images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PRDM5 antibody (ab79016)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PRDM5 antibody (ab79016)
      ab79016, at 5 µg/ml, staining PRDM5 in formalin-fixed, paraffin-embedded human colon tissue by Immunohistochemistry.

    References for Anti-PRDM5 antibody (ab79016)

    ab79016 has not yet been referenced specifically in any publications.

    Publishing research using ab79016? Please let us know so that we can cite the reference in this datasheet.

    Product Wall

    Submit an abreview for ab79016 Submit a question for ab79016

    2 Abreviews or Q&A

    10
    Votes: Highest First

    Immunocytochemistry/ Immunofluorescence abreview for Anti-PRDM5 antibody

     Excellent
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Sample
    Human Cell (Human corneal endothelial cells (HCEC-12))
    Specification
    Human corneal endothelial cells (HCEC-12)
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More
    abreview image
    Username

    Mr. Kostadin Rolev

    Verified customer

    Submitted Apr 29 2014

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PRDM5 antibody

     Excellent
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Peroxide block (3-4% Hydrogen peroxide) as blocking agent for 5 minute(s) · Concentration: 3% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EDTA based buffer - Epitope Retreival solution 2 (pH 9) for 20 min Cat No:AR9640
    Sample
    Human Tissue sections (Human corneas)
    Specification
    Human corneas
    Permeabilization
    Yes - Heat mediated at 100oC
    Fixative
    Formaldehyde
    Read More
    abreview image
    Username

    Mr. Kostadin Rolev

    Verified customer

    Submitted Feb 07 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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