Customer reviews and FAQs

Overview

  • Product nameAnti-PRDM5 antibody
    See all PRDM5 primary antibodies
  • Description
    Rabbit polyclonal to PRDM5
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a portion of amino acids 100-200 of human PRDM5 protein

  • Positive control
    • Human colon tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab79016 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 73 kDa.
IHC-P Use a concentration of 5 µg/ml.

Target

  • FunctionSequence-specific DNA-binding transcription factor. Represses transcription at least in part by recruitment of the histone methyltransferase EHMT2/G9A and histone deacetylases such as HDAC1. Regulates hematopoiesis-associated protein-coding and microRNA (miRNA) genes.
  • Sequence similaritiesContains 16 C2H2-type zinc fingers.
    Contains 1 SET domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
    • Alternative names
      • BCS2 antibody
      • PFM 2 antibody
      • PFM2 antibody
      • PR domain containing 5 antibody
      • PR domain containing protein 5 antibody
      • PR domain zinc finger protein 5 antibody
      • PR domain-containing protein 5 antibody
      • PRDM 5 antibody
      • PRDM5 antibody
      • PRDM5 protein antibody
      • PRDM5_HUMAN antibody
      see all

    Anti-PRDM5 antibody images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PRDM5 antibody (ab79016)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PRDM5 antibody (ab79016)
      ab79016, at 5 µg/ml, staining PRDM5 in formalin-fixed, paraffin-embedded human colon tissue by Immunohistochemistry.

    References for Anti-PRDM5 antibody (ab79016)

    ab79016 has not yet been referenced specifically in any publications.

    Publishing research using ab79016? Please let us know so that we can cite the reference in this datasheet.

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    Submit an abreview for ab79016 Submit a question for ab79016

    2 Abreviews or Q&A

    10
    Votes: Highest First

    Immunocytochemistry/ Immunofluorescence abreview for Anti-PRDM5 antibody

     Excellent
    Abreviews
    Application Immunocytochemistry/ Immunofluorescence
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Sample Human Cell (Human corneal endothelial cells (HCEC-12))
    Specification Human corneal endothelial cells (HCEC-12)
    Permeabilization No
    Fixative Paraformaldehyde
    Read More
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    Username

    Mr. Kostadin Rolev

    Verified customer

    Submitted Apr 29 2014

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PRDM5 antibody

     Excellent
    Abreviews
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step Peroxide block (3-4% Hydrogen peroxide) as blocking agent for 5 minute(s) · Concentration: 3% · Temperature: 25°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA based buffer - Epitope Retreival solution 2 (pH 9) for 20 min Cat No:AR9640
    Sample Human Tissue sections (Human corneas)
    Specification Human corneas
    Permeabilization Yes - Heat mediated at 100oC
    Fixative Formaldehyde
    Read More
    abreview image
    Username

    Mr. Kostadin Rolev

    Verified customer

    Submitted Feb 07 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"