Overview

  • Product name
    Anti-PRDM5 antibody
  • Description
    Rabbit polyclonal to PRDM5
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a portion of amino acids 100-200 of human PRDM5 protein

  • Positive control
    • Human colon tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab79016 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 73 kDa.
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function
    Sequence-specific DNA-binding transcription factor. Represses transcription at least in part by recruitment of the histone methyltransferase EHMT2/G9A and histone deacetylases such as HDAC1. Regulates hematopoiesis-associated protein-coding and microRNA (miRNA) genes.
  • Sequence similarities
    Contains 16 C2H2-type zinc fingers.
    Contains 1 SET domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
    • Alternative names
      • BCS2 antibody
      • PFM 2 antibody
      • PFM2 antibody
      • PR domain containing 5 antibody
      • PR domain containing protein 5 antibody
      • PR domain zinc finger protein 5 antibody
      • PR domain-containing protein 5 antibody
      • PRDM 5 antibody
      • PRDM5 antibody
      • PRDM5 protein antibody
      • PRDM5_HUMAN antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PRDM5 antibody (ab79016)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PRDM5 antibody (ab79016)
      ab79016, at 5 µg/ml, staining PRDM5 in formalin-fixed, paraffin-embedded human colon tissue by Immunohistochemistry.

    References

    This product has been referenced in:

    See 1 Publication for this product

    Publishing research using ab79016? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    2 reviews or Q&A

    10
    Votes: Highest First

    Immunocytochemistry/ Immunofluorescence abreview for Anti-PRDM5 antibody

     Excellent
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Sample
    Human Cell (Human corneal endothelial cells (HCEC-12))
    Specification
    Human corneal endothelial cells (HCEC-12)
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More
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    Username

    Mr. Kostadin Rolev

    Verified customer

    Submitted Apr 29 2014

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PRDM5 antibody

     Excellent
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Peroxide block (3-4% Hydrogen peroxide) as blocking agent for 5 minute(s) · Concentration: 3% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EDTA based buffer - Epitope Retreival solution 2 (pH 9) for 20 min Cat No:AR9640
    Sample
    Human Tissue sections (Human corneas)
    Specification
    Human corneas
    Permeabilization
    Yes - Heat mediated at 100oC
    Fixative
    Formaldehyde
    Read More
    abreview image
    Username

    Mr. Kostadin Rolev

    Verified customer

    Submitted Feb 07 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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