Anti-pre-mRNA cleavage factor I (59 kDa subunit) antibody (ab50965)

Overview

  • Product name
    Anti-pre-mRNA cleavage factor I (59 kDa subunit) antibody
    See all pre-mRNA cleavage factor I (59 kDa subunit) primary antibodies
  • Description
    Rabbit polyclonal to pre-mRNA cleavage factor I (59 kDa subunit)
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog
  • Immunogen

    Synthetic peptide within Human pre-mRNA cleavage factor I (59 kDa subunit) (C terminal). The exact sequence is proprietary.
    Sequence:

    SRERSPSRSRESSRRHRDLLHNEDRHDDYFQERNREHERHRDRERDRHH

  • Positive control
    • WB: HepG2 cell lysate IHC-P: Human lung tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50965 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 52 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

Target

  • Function
    Component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3' processing. Binds to cleavage and polyadenylation RNA substrates.
  • Sequence similarities
    Belongs to the RRM CPSF6/7 family.
    Contains 1 RRM (RNA recognition motif) domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CFIm59 antibody
    • Cleavage and polyadenylation specificity factor 59 kDa subunit antibody
    • Cleavage and polyadenylation specificity factor 7 antibody
    • Cleavage and polyadenylation specificity factor subunit 7 antibody
    • CPSF 59 kDa subunit antibody
    • Cpsf7 antibody
    • CPSF7_HUMAN antibody
    • FLJ12529 pre mRNA cleavage factor I 59 kDa subunit antibody
    • FLJ39024 antibody
    • MGC9315 antibody
    • Pre mRNA cleavage factor Im 59 kDa subunit antibody
    • Pre-mRNA cleavage factor Im 59 kDa subunit antibody
    see all

Images

  • Lane 1 :
    Lane 2 : Anti-pre-mRNA cleavage factor I (59 kDa subunit) antibody (ab50965) at 1.25 µg/ml

    Lane 1 : Marker
    Lane 2 : HepG2 cell lysate at 10 µg

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50,000


    Predicted band size : 52 kDa
    Observed band size : 54 kDa (why is the actual band size different from the predicted?)
  • ab50965, at a concentration of 4-8 µg/ml, staining pre mRNA cleavage factor I (59 kDa subunit) in paraffin embedded human lung tissue sections by Immunohistochemistry. Cells with positive label are Aveolar cells (indicated by arrows). Magnification: 400x.

References

This product has been referenced in:
  • Kim S  et al. Evidence that cleavage factor Im is a heterotetrameric protein complex controlling alternative polyadenylation. Genes Cells 15:1003-13 (2010). Read more (PubMed: 20695905) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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