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Rabbit polyclonal to pre-mRNA cleavage factor I (59 kDa subunit)
Predicted to work with:
Mouse, Rat, Dog
Synthetic peptide within Human pre-mRNA cleavage factor I (59 kDa subunit) (C terminal). The exact sequence is proprietary.
WB: HepG2 cell lysate
IHC-P: Human lung tissue
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Protein A purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1.25 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 52 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use a concentration of 4 - 8 µg/ml.
Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/62500.
Component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3' processing. Binds to cleavage and polyadenylation RNA substrates.
Belongs to the RRM CPSF6/7 family.
Contains 1 RRM (RNA recognition motif) domain.
Information by UniProt
Cleavage and polyadenylation specificity factor 59 kDa subunit antibody
Cleavage and polyadenylation specificity factor 7 antibody
Western blot - pre-mRNA cleavage factor I (59 kDa subunit) antibody (ab50965)
Lane 2 :
Anti-pre-mRNA cleavage factor I (59 kDa subunit) antibody (ab50965) at 1.25 µg/ml
Lane 1 :
Lane 2 :
HepG2 cell lysate at 10 µg
Secondary Lane 2 :
HRP conjugated anti-Rabbit IgG at 1/50,000
Predicted band size:
Observed band size:
54 kDa (
why is the actual band size different from the predicted?
Immunohistochemistry (Paraffin-embedded sections) - pre-mRNA cleavage factor I (59 kDa subunit) antibody (ab50965)
ab50965, at a concentration of 4-8 µg/ml, staining pre mRNA cleavage factor I (59 kDa subunit) in
paraffin embedded human lung tissue sections by Immunohistochemistry. Cells with positive label are Aveolar cells (indicated by arrows). Magnification: 400x.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"