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Rabbit polyclonal to pre-mRNA cleavage factor I (59 kDa subunit)
Predicted to work with:
Mouse, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Orangutan
Immunogen corresponds to a region between residue 1 and 50 of Human pre-mRNA cleavage factor I (59 kDa subunit) (NP_079087.2)
HeLa whole cell lysate or 293T cell lysate
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 52 kDa.
Use at 2-5 µg/mg of lysate.
Component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3' processing. Binds to cleavage and polyadenylation RNA substrates.
Belongs to the RRM CPSF6/7 family.
Contains 1 RRM (RNA recognition motif) domain.
Information by UniProt
Cleavage and polyadenylation specificity factor 59 kDa subunit antibody
Cleavage and polyadenylation specificity factor 7 antibody
Western blot - Anti-pre-mRNA cleavage factor I (59 kDa subunit) antibody (ab99348)
All lanes : Anti-pre-mRNA cleavage factor I (59 kDa subunit) antibody (ab99348) at 0.04 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Developed using the ECL technique. Predicted band size: 52 kDa Exposure time: 3 minutes
Immunoprecipitation - Anti-pre-mRNA cleavage factor I (59 kDa subunit) antibody (ab99348)
Detection of pre-mRNA cleavage factor I (59 kDa subunit) by Western Blot of Immunprecipitate.
ab99348 at 1µg/ml staining pre-mRNA cleavage factor I (59 kDa subunit) in HeLa whole cell lysate immunoprecipitated using ab99348 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"