Anti-pre-mRNA cleavage factor I (59 kDa subunit) antibody (ab99348)

Overview

  • Product name
    Anti-pre-mRNA cleavage factor I (59 kDa subunit) antibody
    See all pre-mRNA cleavage factor I (59 kDa subunit) primary antibodies
  • Description
    Rabbit polyclonal to pre-mRNA cleavage factor I (59 kDa subunit)
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Orangutan
  • Immunogen

    Immunogen corresponds to a region between residue 1 and 50 of Human pre-mRNA cleavage factor I (59 kDa subunit) (NP_079087.2)

  • Positive control
    • HeLa whole cell lysate or 293T cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab99348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 52 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    Component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3' processing. Binds to cleavage and polyadenylation RNA substrates.
  • Sequence similarities
    Belongs to the RRM CPSF6/7 family.
    Contains 1 RRM (RNA recognition motif) domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CFIm59 antibody
    • Cleavage and polyadenylation specificity factor 59 kDa subunit antibody
    • Cleavage and polyadenylation specificity factor 7 antibody
    • Cleavage and polyadenylation specificity factor subunit 7 antibody
    • CPSF 59 kDa subunit antibody
    • Cpsf7 antibody
    • CPSF7_HUMAN antibody
    • FLJ12529 pre mRNA cleavage factor I 59 kDa subunit antibody
    • FLJ39024 antibody
    • MGC9315 antibody
    • Pre mRNA cleavage factor Im 59 kDa subunit antibody
    • Pre-mRNA cleavage factor Im 59 kDa subunit antibody
    see all

Images

  • All lanes : Anti-pre-mRNA cleavage factor I (59 kDa subunit) antibody (ab99348) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 52 kDa


    Exposure time: 3 minutes
  • Detection of pre-mRNA cleavage factor I (59 kDa subunit) by Western Blot of Immunprecipitate. ab99348 at 1µg/ml staining pre-mRNA cleavage factor I (59 kDa subunit) in HeLa whole cell lysate immunoprecipitated using ab99348 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.

References

ab99348 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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