Anti-pre-mRNA cleavage factor I (59 kDa subunit) antibody (ab99348)

Overview

  • Product nameAnti-pre-mRNA cleavage factor I (59 kDa subunit) antibody
    See all pre-mRNA cleavage factor I (59 kDa subunit) primary antibodies
  • Description
    Rabbit polyclonal to pre-mRNA cleavage factor I (59 kDa subunit)
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Orangutan
  • Immunogen

    Immunogen corresponds to a region between residue 1 and 50 of Human pre-mRNA cleavage factor I (59 kDa subunit) (NP_079087.2)

  • Positive control
    • HeLa whole cell lysate or 293T cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab99348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 52 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • FunctionComponent of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3' processing. Binds to cleavage and polyadenylation RNA substrates.
  • Sequence similaritiesBelongs to the RRM CPSF6/7 family.
    Contains 1 RRM (RNA recognition motif) domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CFIm59 antibody
    • Cleavage and polyadenylation specificity factor 59 kDa subunit antibody
    • Cleavage and polyadenylation specificity factor 7 antibody
    • Cleavage and polyadenylation specificity factor subunit 7 antibody
    • CPSF 59 kDa subunit antibody
    • Cpsf7 antibody
    • CPSF7_HUMAN antibody
    • FLJ12529 pre mRNA cleavage factor I 59 kDa subunit antibody
    • FLJ39024 antibody
    • MGC9315 antibody
    • Pre mRNA cleavage factor Im 59 kDa subunit antibody
    • Pre-mRNA cleavage factor Im 59 kDa subunit antibody
    see all

Anti-pre-mRNA cleavage factor I (59 kDa subunit) antibody images

  • All lanes : Anti-pre-mRNA cleavage factor I (59 kDa subunit) antibody (ab99348) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 52 kDa


    Exposure time : 3 minutes
  • Detection of pre-mRNA cleavage factor I (59 kDa subunit) by Western Blot of Immunprecipitate. ab99348 at 1µg/ml staining pre-mRNA cleavage factor I (59 kDa subunit) in HeLa whole cell lysate immunoprecipitated using ab99348 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.

References for Anti-pre-mRNA cleavage factor I (59 kDa subunit) antibody (ab99348)

ab99348 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab99348.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"