Human prealbumin purified from plasma.
Our Abpromise guarantee covers the use of ab9015 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|Other||Use at an assay dependent concentration.|
|RID||Use at an assay dependent concentration.|
|Immunoelectrophoresis||Use at an assay dependent concentration.|
|Double Immunodiffusion||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration.
The use of 3% PEG 6000 with 1.2% agarose in a suitable buffer (such as TBE or Tris-barbital pH >8.2) is recommended.
|ICC/IF||Use a concentration of 5 µg/ml.|
ICC/IF image of ab9015 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab9015 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 donkey anti- sheep (ab96939) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.