Recombinant Anti-Prealbumin antibody [EP2929Y] (ab75815)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2929Y] to Prealbumin
- Suitable for: IP, Flow Cyt (Intra), WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Prealbumin antibody [EP2929Y]
See all Prealbumin primary antibodies -
Description
Rabbit monoclonal [EP2929Y] to Prealbumin -
Host species
Rabbit -
Tested applications
Suitable for: IP, Flow Cyt (Intra), WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human heart, placenta and brain lysates; human liver tissue. IP: Human heart lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP2929Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab75815 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
1/50.
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Flow Cyt (Intra) |
1/50.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB | (2) |
1/2500 - 1/5000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).
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IHC-P | (2) |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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IP
1/50. |
Flow Cyt (Intra)
1/50. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/2500 - 1/5000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain. -
Tissue specificity
Detected in serum and cerebrospinal fluid (at protein level). Highly expressed in choroid plexus epithelial cells. Detected in retina pigment epithelium and liver. -
Involvement in disease
Defects in TTR are the cause of amyloidosis transthyretin-related (AMYL-TTR) [MIM:105210]. A hereditary generalized amyloidosis due to transthyretin amyloid deposition. Protein fibrils can form in different tissues leading to amyloid polyneuropathies, amyloidotic cardiomyopathy, carpal tunnel syndrome, systemic senile amyloidosis. The disease includes leptomeningeal amyloidosis that is characterized by primary involvement of the central nervous system. Neuropathologic examination shows amyloid in the walls of leptomeningeal vessels, in pia arachnoid, and subpial deposits. Some patients also develop vitreous amyloid deposition that leads to visual impairment (oculoleptomeningeal amyloidosis). Clinical features include seizures, stroke-like episodes, dementia, psychomotor deterioration, variable amyloid deposition in the vitreous humor.
Defects in TTR are a cause of hyperthyroxinemia dystransthyretinemic euthyroidal (HTDE) [MIM:145680]. It is a condition characterized by elevation of total and free thyroxine in healthy, euthyroid persons without detectable binding protein abnormalities.
Defects in TTR are a cause of carpal tunnel syndrome type 1 (CTS1) [MIM:115430]. It is a condition characterized by entrapment of the median nerve within the carpal tunnel. Symptoms include burning pain and paresthesias involving the ventral surface of the hand and fingers which may radiate proximally. Impairment of sensation in the distribution of the median nerve and thenar muscle atrophy may occur. This condition may be associated with repetitive occupational trauma, wrist injuries, amyloid neuropathies, rheumatoid arthritis. -
Sequence similarities
Belongs to the transthyretin family. -
Domain
Each monomer has two 4-stranded beta sheets and the shape of a prolate ellipsoid. Antiparallel beta-sheet interactions link monomers into dimers. A short loop from each monomer forms the main dimer-dimer interaction. These two pairs of loops separate the opposed, convex beta-sheets of the dimers to form an internal channel. -
Cellular localization
Secreted. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 7276 Human
- Omim: 176300 Human
- SwissProt: P02766 Human
- Unigene: 427202 Human
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Alternative names
- Amyloid polyneuropathy antibody
- Amyloidosis I antibody
- ATTR antibody
see all
Images
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All lanes : Anti-Prealbumin antibody [EP2929Y] (ab75815) at 1/5000 dilution
Lane 1 : human heart lysate
Lane 2 : human placenta lysate
Lane 3 : human brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa -
ab75815 at 1/250 dilution staining Prealbumin in human liver by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Purified ab75815 at 1/50 dilution (2µg) immunoprecipitating Prealbumin in Human heart lysate.
Lane 1 (input): Human heart lysate 10µg
Lane 2 (+): ab75815 + Human heart lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab75815 in Human heart lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 16 kDa -
Intracellular Flow Cytometry analysis of HepG2 cells labelling Prealbumin with ab75815 at a dilution of 1/50 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Green - Isotype control, rabbit monoclonal IgG.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab75815 has been referenced in 3 publications.
- Cheng W et al. Growth differentiation factor 1-induced tumour plasticity provides a therapeutic window for immunotherapy in hepatocellular carcinoma. Nat Commun 12:7142 (2021). PubMed: 34880251
- Keeratichamroen S et al. Identification of potential cervical cancer serum biomarkers in Thai patients. Oncol Lett 19:3815-3826 (2020). PubMed: 32391095
- Katsuda T et al. Generation of human hepatic progenitor cells with regenerative and metabolic capacities from primary hepatocytes. Elife 8:N/A (2019). PubMed: 31393263