Anti-PREB antibody (ab3422)

Overview

  • Product name
  • Description
    Rabbit polyclonal to PREB
  • Specificity
    Detects recombinant mouse PREB (Prolactin Regulatory Element Binding protein).
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Recombinant fragment corresponding to Mouse PREB.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).

Target

  • Function
    Was first identified based on its probable role in the regulation of pituitary gene transcription. Binds to the prolactin gene (PRL) promoter and seems to activate transcription (By similarity). Guanine nucleotide exchange factor that activates SARA2. Required for the formation of COPII transport vesicles from the ER.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Contains 3 WD repeats.
  • Cellular localization
    Nucleus. Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Mammalian guanine nucleotide exchange factor mSec12 antibody
    • Preb antibody
    • PREB_HUMAN antibody
    • Prolactin regulatory binding element protein antibody
    • Prolactin regulatory element binding antibody
    • Prolactin regulatory element binding protein antibody
    • Prolactin regulatory element-binding protein antibody
    • SEC12 antibody
    see all

Images



  • Predicted band size : 45 kDa

    Western blot detection of recombinant mouse PREB using ab3422.

  • ab3422 staining PREB in mouse skeletal tissue sections by Immunohistochemistry (Frozen sections)

    Tissue was fixed with acetone, blocked with 10% serum for 1 hour at 22°C and permeabilized with 1% Triton X-100. Samples were incubated with primary antibody (1/50 in 10% serum) for 12 hours at 4°C. A Rhodamine red-x conjugated donkey anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

References

ab3422 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Skeletal Muscle)
Specification
Skeletal Muscle
Fixative
Acetone
Permeabilization
Yes - 1% Triton-X
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Username

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Verified customer

Submitted Jun 28 2012

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I am very pleased to hear you would like to accept our offer and test ab3422 in IHC-Fr on mouse tissue. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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