Overview

  • Product nameAnti-PREB antibody
    See all PREB primary antibodies
  • Description
    Rabbit polyclonal to PREB
  • SpecificityThis antibody is specific for PREB.
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A region within synthetic peptide: RVENLQAVQT DFSSDPLQKV VCFNHDNTLL ATGGTDGYVR VWKVPSLEKV, corresponding to amino acids 140-189 of Human PREB

  • Positive control
    • Raji cell lysate and human heart tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab42501 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 41, 30 kDa (predicted molecular weight: 45 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/312500.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • FunctionWas first identified based on its probable role in the regulation of pituitary gene transcription. Binds to the prolactin gene (PRL) promoter and seems to activate transcription (By similarity). Guanine nucleotide exchange factor that activates SARA2. Required for the formation of COPII transport vesicles from the ER.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesContains 3 WD repeats.
  • Cellular localizationNucleus. Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Mammalian guanine nucleotide exchange factor mSec12 antibody
    • Preb antibody
    • PREB_HUMAN antibody
    • Prolactin regulatory binding element protein antibody
    • Prolactin regulatory element binding antibody
    • Prolactin regulatory element binding protein antibody
    • Prolactin regulatory element-binding protein antibody
    • SEC12 antibody
    see all

Anti-PREB antibody images

  • Anti-PREB antibody (ab42501) at 0.5 µg/ml + Raji cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 45 kDa
    Observed band size : 41& 30 kDa (why is the actual band size different from the predicted?)
  • ab42501 at 4µg/ml staining PREB in human myocardial cells.

References for Anti-PREB antibody (ab42501)

ab42501 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"