For the best experience on the Abcam website please upgrade to a modern browser such as
Goat polyclonal to PREI3
ab114057 is expected to recognize all three reported isoforms (NP_056202.2; NP_955776.1; NP_001094289.1).
Mouse, Rat, Human
Predicted to work with:
Chicken, Cow, Dog, Pig, Xenopus laevis
Synthetic peptide corresponding to Human PREI3 aa 137-151 (internal sequence).
Human Brain (Frontal Cortex), Mouse and Rat Brain lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
Immunogen affinity purified
ab114057 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Antibody detection limit dilution 1:4,000.
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Belongs to the MOB1/phocein family.
Phosphorylated on serine residues.
Cytoplasm > perinuclear region. Membrane. Golgi apparatus > Golgi stack membrane. In a perinuclear punctate pattern. Associated with membranes and the Golgi stacks.
Information by UniProt
CGI 95 antibody
class II mMOB1 antibody
Western blot - Anti-PREI3 antibody (ab114057)
All lanes : Anti-PREI3 antibody (ab114057) at 0.3 µg/ml Lane 1 : Mouse Brain lysate Lane 2 : Rat Brain lysate Lysates/proteins at 35 µg per lane. Predicted band size: 26 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PREI3 antibody (ab114057)
ab114057 (5µg/ml) shows staining of PREI3 in paraffin embedded Human Cerebral Cortex following steamed antigen retrieval with citrate buffer pH 6 and AP-staining. This shows cytoplasm staining in the neuronal bodies.
Western blot - PREI3 antibody (ab114057)
Anti-PREI3 antibody (ab114057) at 0.3 µg/ml + Human Brain, Frontal Cortex lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique. Predicted band size: 26 kDa Primary incubation was 1 hour.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"