Overview

  • Product nameAnti-PREI3 antibody
    See all PREI3 primary antibodies
  • Description
    Goat polyclonal to PREI3
  • Specificityab114057 is expected to recognize all three reported isoforms (NP_056202.2; NP_955776.1; NP_001094289.1).
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow, Dog, Pig, Xenopus laevis
  • Immunogen

    Synthetic peptide corresponding to Human PREI3 aa 137-151 (internal sequence).
    Sequence:

    C-NSNKYFPSRVSIKES


    Database link: Q9Y3A3

  • Positive control
    • Human Brain (Frontal Cortex), Mouse and Rat Brain lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab114057 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Antibody detection limit dilution 1:4,000.

WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Anti-PREI3 antibody images

  • All lanes : Anti-PREI3 antibody (ab114057) at 0.3 µg/ml

    Lane 1 : Mouse Brain lysate
    Lane 2 : Rat Brain lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 26 kDa
  • ab114057 (5µg/ml) shows staining of PREI3 in paraffin embedded Human Cerebral Cortex following steamed antigen retrieval with citrate buffer pH 6 and AP-staining. This shows cytoplasm staining in the neuronal bodies.

  • Anti-PREI3 antibody (ab114057) at 0.3 µg/ml + Human Brain, Frontal Cortex lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 26 kDa
    Primary incubation was 1 hour.

References for Anti-PREI3 antibody (ab114057)

ab114057 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"