Overview

  • Product nameAnti-Presenilin 1 antibody
    See all Presenilin 1 primary antibodies
  • Description
    Rabbit polyclonal to Presenilin 1
  • Tested applicationsSuitable for: IHC-P, WB, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Dog, Pig, Non Human Primates
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human Presenilin 1.

    (Peptide available as ab71179.)

  • Positive control
    • This antibody gave a positive signal in Mouse Kidney Tissue lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab65293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
IHC-FoFr 1/300.

Target

  • FunctionProbable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Requires the other members of the gamma-secretase complex to have a protease activity. May play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. Stimulates cell-cell adhesion though its association with the E-cadherin/catenin complex. Under conditions of apoptosis or calcium influx, cleaves E-cadherin promoting the disassembly of the E-cadherin/catenin complex and increasing the pool of cytoplasmic beta-catenin, thus negatively regulating Wnt signaling. May also play a role in hematopoiesis.
  • Tissue specificityExpressed in a wide range of tissues including various regions of the brain, liver, spleen and lymph nodes.
  • Involvement in diseaseDefects in PSEN1 are a cause of Alzheimer disease type 3 (AD3) [MIM:607822]. AD3 is a familial early-onset form of Alzheimer disease. Alzheimer disease is a neurodegenerative disorder characterized by progressive dementia, loss of cognitive abilities, and deposition of fibrillar amyloid proteins as intraneuronal neurofibrillary tangles, extracellular amyloid plaques and vascular amyloid deposits. The major constituent of these plaques is the neurotoxic amyloid-beta-APP 40-42 peptide (s), derived proteolytically from the transmembrane precursor protein APP by sequential secretase processing. The cytotoxic C-terminal fragments (CTFs) and the caspase-cleaved products such as C31 derived from APP, are also implicated in neuronal death.
    Defects in PSEN1 are a cause of frontotemporal dementia [MIM:600274].
    Defects in PSEN1 are the cause of cardiomyopathy dilated type 1U (CMD1U) [MIM:613694]. It is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in PSEN1 are the cause of acne inversa familial type 3 (ACNIF3) [MIM:613737]. A chronic relapsing inflammatory disease of the hair follicles characterized by recurrent draining sinuses, painful skin abscesses, and disfiguring scars. Manifestations typically appear after puberty.
  • Sequence similaritiesBelongs to the peptidase A22A family.
  • DomainThe PAL motif is required for normal active site conformation.
  • Post-translational
    modifications
    Heterogeneous proteolytic processing generates N-terminal (NTF) and C-terminal (CTF) fragments of approximately 35 and 20 kDa, respectively. During apoptosis, the C-terminal fragment (CTF) is further cleaved by caspase-3 to produce the fragment, PS1-CTF12.
    After endoproteolysis, the C-terminal fragment (CTF) is phosphorylated on serine residues by PKA and/or PKC. Phosphorylation on Ser-346 inhibits endoproteolysis.
  • Cellular localizationEndoplasmic reticulum membrane. Golgi apparatus membrane. Cell surface. Bound to NOTCH1 also at the cell surface. Colocalizes with CDH1/2 at sites of cell-cell contact. Colocalizes with CTNNB1 in the endoplasmic reticulum and the proximity of the plasma membrane. Also present in azurophil granules of neutrophils.
  • Information by UniProt
  • Database links
  • Alternative names
    • AD3 antibody
    • Ad3h antibody
    • FAD antibody
    • Homo Sapiens Clone CC44 Senilin 1 antibody
    • Presenilin-1 CTF12 antibody
    • Protein S182 antibody
    • PS 1 antibody
    • PS-1 antibody
    • PS1-CTF12 antibody
    • PSEN1 antibody
    • PSN1_HUMAN antibody
    • PSNL1 antibody
    • S182 antibody
    see all

Anti-Presenilin 1 antibody images

  • Immunohistochemistical detection of Presenilin 1 using ab65293 on PFA perfusion fixed frozen rat brain 30um sections. Primary Antibody Dilution 1/300 for 18 hours @ 20°C in PBS + 0.3 % TritonX100. Secondary Antibody: Goat anti-rabbit Alexa Fluor® 488 (1/1000). ab65293 was used on 30um sections of rat brain tissue in free floating IHC. The antibody produced punctate staining in the plasma membrane and processes of neurons in many brain areas. The image submitted shows staining in the cerebral cortex.

    See Abreview

  • ab65293 staining Presenilin 1 in human kidney. The sectioned were ixed in formaldehyde and subjected to heat mediated antigen retrieval using citric acid (pH 6). The tissue was then blocked with 1% BSA for 10 mins at 21 °C. Teh antibody was diluted to 1/500 using TBS/BSA/Azide and incubated for 2 hours at 21°C.

    See Abreview

  • Anti-Presenilin 1 antibody (ab65293) at 1 µg/ml + Brain (Mouse) Tissue Lysate at 20 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 53 kDa
    Observed band size : 53 kDa
    Additional bands at : 14 kDa,48 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes

References for Anti-Presenilin 1 antibody (ab65293)

This product has been referenced in:
  • Belal C  et al. The homocysteine-inducible endoplasmic reticulum (ER) stress protein Herp counteracts mutant a-synuclein-induced ER stress via the homeostatic regulation of ER-resident calcium release channel proteins. Hum Mol Genet 21:963-77 (2012). WB ; Human . Read more (PubMed: 22045699) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Kidney)
Specification Kidney
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Sep 06 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (kidney)
Specification kidney
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Aug 16 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain sections)
Specification Brain sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

Submitted May 26 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"