Overview

  • Product nameAnti-Presenilin 2 [APS 26] antibodySee all Presenilin 2 primary antibodies ...
  • Description
    Mouse monoclonal [APS 26] to Presenilin 2
  • SpecificityNo cross-reactivity is seen with presenilin 1.
  • Tested applicationsICC/IF, IP, ICC, IHC-P, WB, ELISA more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Pig, Non Human Primates
  • Immunogen

    Synthetic peptide corresponding to Human Presenilin 2 aa 317-334.
    Sequence: LPYDPEMEEDSYDSFGEP

  • Positive control
    • RAW cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab15549 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF Use at an assay dependent concentration. PubMed: 21163940
IP Use at an assay dependent concentration. PubMed: 21163940
ICC Use at an assay dependent concentration.
IHC-P 1/20.
WB Use a concentration of 12 µg/ml. Detects a band of approximately 20, 45 kDa.
ELISA Use a concentration of 4.5 µg/ml.

Target

  • FunctionProbable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Requires the other members of the gamma-secretase complex to have a protease activity. May play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. May function in the cytoplasmic partitioning of proteins.
  • Tissue specificityIsoform 1 is seen in the placenta, skeletal muscle and heart while isoform 2 is seen in the heart, brain, placenta, liver, skeletal muscle and kidney.
  • Involvement in diseaseDefects in PSEN2 are the cause of Alzheimer disease type 4 (AD4) [MIM:606889]. AD is an autosomal dominant Alzheimer disease. Alzheimer disease is a neurodegenerative disorder characterized by progressive dementia, loss of cognitive abilities, and deposition of fibrillar amyloid proteins as intraneuronal neurofibrillary tangles, extracellular amyloid plaques and vascular amyloid deposits. The major constituent of these plaques is the neurotoxic amyloid-beta-APP 40-42 peptide (s), derived proteolytically from the transmembrane precursor protein APP by sequential secretase processing. The cytotoxic C-terminal fragments (CTFs) and the caspase-cleaved products such as C31 derived from APP, are also implicated in neuronal death.
    Defects in PSEN2 are the cause of cardiomyopathy dilated type 1V (CMD1V) [MIM:613697]. It is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
  • Sequence similaritiesBelongs to the peptidase A22A family.
  • DomainThe PAL motif is required for normal active site conformation.
  • Post-translational
    modifications
    Heterogeneous proteolytic processing generates N-terminal and C-terminal fragments.
    Phosphorylated on serine residues.
  • Cellular localizationEndoplasmic reticulum membrane. Golgi apparatus membrane.
  • Target information above from: UniProt accession P49810 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • AD 4 antibody
    • AD 4 antibody
    • AD3L antibody
    • AD3L antibody
    • AD3LP antibody
    • AD3LP antibody
    • AD4 antibody
    • AD4 antibody
    • AD5 antibody
    • AD5 antibody
    • Alzheimer disease 4 antibody
    • Alzheimer disease 4 antibody
    • Alzheimer Disease Familial Type 4 antibody
    • Alzheimer Disease Familial Type 4 antibody
    • Alzheimer's disease 3 like antibody
    • Alzheimer's disease 3 like antibody
    • CMD1V antibody
    • E5 1 antibody
    • E5 1 antibody
    • E5-1 antibody
    • OTTHUMP00000035671 antibody
    • OTTHUMP00000035672 antibody
    • OTTHUMP00000228286 antibody
    • OTTHUMP00000228288 antibody
    • Presenilin 2 (Alzheimer disease 4) antibody
    • Presenilin 2 antibody
    • Presenilin-2 CTF subunit antibody
    • PS 2 antibody
    • PS 2 antibody
    • PS-2 antibody
    • PS2 antibody
    • PS2 antibody
    • PSEN 2 antibody
    • PSEN 2 antibody
    • PSEN2 antibody
    • PSEN2 antibody
    • PSN2_HUMAN antibody
    • PSNL2 antibody
    • PSNL2 antibody
    • STM-2 antibody
    • STM2 antibody
    • STM2 antibody
    see all

Anti-Presenilin 2 [APS 26] antibody images

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling Presenilin 2 (green) with ab15549 at 1/20. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.

  • Immunocytochemistry/Immunofluorescence analysis of A2058 cells labeling Presenilin 2 (green) with ab15549 at 1/20. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.

  • IF staining Presenilin 2 in Mouse fibroblasts using ab15549 

  • Immunohistochemistry was performed on normal biopsies of deparaffinized Human kidney tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Presenilin 2 ab15549 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

References for Anti-Presenilin 2 [APS 26] antibody (ab15549)

This product has been referenced in:
  • Rivabene R  et al. Thapsigargin affects presenilin-2 but not presenilin-1 regulation in SK-N-BE cells. Exp Biol Med (Maywood) 239:213-24 (2014). Human . Read more (PubMed: 24363250) »
  • Soler-López M  et al. Interactome mapping suggests new mechanistic details underlying Alzheimer's disease. Genome Res 21:364-76 (2011). WB, ICC/IF, IP ; Human . Read more (PubMed: 21163940) »

See all 2 Publications for this product

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