Overview

  • Product name
  • Description
    Rabbit polyclonal to PREX2
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen, corresponding to amino acids 686-835 of Human PREX2

  • Positive control
    • Human stomach tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121462 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • Function
    Functions as a RAC1 guanine nucleotide exchange factor (GEF), activating Rac proteins by exchanging bound GDP for free GTP. Its activity is synergistically activated by phosphatidylinositol-3,4,5-triphosphate and the beta gamma subunits of heterotrimeric G protein. Mediates the activation of RAC1 in a PI3K-dependent manner. May be an important mediator of Rac signaling, acting directly downstream of both G protein-coupled receptors and phosphoinositide 3-kinase.
  • Tissue specificity
    Isoform 1 is highly expressed in skeletal muscle, heart and placenta, absent from peripheral blood leukocytes. Isoform 2 is expressed in skeletal muscle, kidney, small intestine, and placenta. Isoform 3 is expressed in the heart.
  • Sequence similarities
    Contains 2 DEP domains.
    Contains 1 DH (DBL-homology) domain.
    Contains 2 PDZ (DHR) domains.
    Contains 1 PH domain.
  • Domain
    PH domain confers substrate specificity and recognition. Able to discriminate between RAC1, RHOA, and CDC42.
    DH domain alone was unable to confer substrate specificity and recognition.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4 antibody
    • 5)-dependent Rac exchanger 2 antibody
    • 5-trisphosphate-dependent Rac exchanger 2 protein antibody
    • 6230420N16Rik antibody
    • DEP domain-containing protein 2 antibody
    • DEP.2 antibody
    • DEPDC2 antibody
    • FLJ12987 antibody
    • P-Rex2 antibody
    • Phosphatidylinositol 3 antibody
    • phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 antibody
    • phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein antibody
    • phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2 antibody
    • PREX2 antibody
    • PREX2_HUMAN antibody
    • PtdIns(3 antibody
    • PtdIns(3,4,5)-dependent Rac exchanger 2 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nuclei but not nucleoli. Recommended concentration of ab121462 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121462 at a 1/725 dilution staining PREX2 in Human stomach tissue (paraffin embedded) by immunohistochemistry.

References

This product has been referenced in:
  • He S  et al. Upregulation of PREX2 promotes the proliferation and migration of hepatocellular carcinoma cells via PTEN-AKT signaling. Oncol Lett 11:2223-2228 (2016). Human . Read more (PubMed: 26998152) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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