Overview

  • Product nameAnti-Prickle 2 antibody
    See all Prickle 2 primary antibodies
  • Description
    Rabbit polyclonal to Prickle 2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 700 - 800 of Human Prickle 2.

    (Peptide available as ab90635.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: Y79; WERI.

Properties

Applications

Our Abpromise guarantee covers the use of ab65964 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 96 kDa (predicted molecular weight: 96 kDa).

Target

  • Tissue specificityExpressed in brain, eye and testis. Additionally in fetal brain, adult cartilage, pancreatic islet, gastric cancer and uterus tumors.
  • Involvement in diseaseEpilepsy, progressive myoclonic 5
  • Sequence similaritiesBelongs to the prickle / espinas / testin family.
    Contains 3 LIM zinc-binding domains.
    Contains 1 PET domain.
  • Cellular localizationNucleus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • EPM5 antibody
    • PRIC2 antibody
    • PRIC2_HUMAN antibody
    • Prickle homolog 2 (Drosophila) antibody
    • Prickle planar cell polarity protein 2 antibody
    • prickle-like 2 (Drosophila) antibody
    • prickle-like 2 antibody
    • Prickle-like protein 2 antibody
    • PRICKLE2 antibody
    see all

Anti-Prickle 2 antibody images

  • All lanes : Anti-Prickle 2 antibody (ab65964) at 1 µg/ml

    Lane 1 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
    Lane 2 : WERI (Human Retinoblastoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 96 kDa
    Observed band size : 96 kDa
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 minutes

References for Anti-Prickle 2 antibody (ab65964)

ab65964 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
Sample Mouse Tissue sections (E9.5 mouse embryo section)
Specification E9.5 mouse embryo section
Permeabilization Yes - PBST (PBS/0.1% v/v TritonX100 (sigma))
Fixative Paraformaldehyde
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Submitted Jan 30 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"