This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

Anti-Prickle antibody (ab15577)


  • Product nameAnti-Prickle antibody
    See all Prickle primary antibodies
  • Description
    Rabbit polyclonal to Prickle

    This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »

  • SpecificityImmunogen sequence most homologous with Prickle 1, but is ~65% identical with Prickle 2 sequences, thus some reaction with Prickle 2 may be seen as well as with Prickle 1.
  • Species reactivity

    Predicted to work with: Mouse, Rat, Human, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Xenopus laevis Prickle.

    (Peptide available as ab15578.)


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

Application Abreviews Notes
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ELISA Use at an assay dependent concentration. This antibody gave a positive result in ELISA against the immunizing peptide (ab15578).


Anti-Prickle antibody images

This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

  • ab15577 (1/50) staining Prickle in paraffin-embbeded human uterus (smooth muscle). Tissues underwent formaldehyde fixation, heat mediated antigen retrieval and peroxidase blocking prior to staining of the sections. Please refer to Abreview for further experimental details.

    See Abreview

References for Anti-Prickle antibody (ab15577)

ab15577 has not yet been referenced specifically in any publications.

Product Wall

This antibody was raised against Prickle1.

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Uterus (smooth muscle))
Specification Uterus (smooth muscle)
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C

Mr. Antibody Solutions

Verified customer

Submitted Feb 01 2011

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Bone and muscle)
Specification Bone and muscle
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 09 2008

Thank you for your enquiry. I have performed an alignment of your ascidian prickle sequence against our immunogen sequence. The alignment can be accessed at the following ClustalW link for the next 24 hours.

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