This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

Anti-Prickle antibody (ab15577)

Overview

  • Product name
  • Description
    Rabbit polyclonal to Prickle

    This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »

  • Specificity
    Immunogen sequence most homologous with Prickle 1, but is ~65% identical with Prickle 2 sequences, thus some reaction with Prickle 2 may be seen as well as with Prickle 1.
  • Species reactivity

    Predicted to work with: Mouse, Rat, Human, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Xenopus laevis Prickle.

    (Peptide available as ab15578.)

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

Application Abreviews Notes
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ELISA Use at an assay dependent concentration. This antibody gave a positive result in ELISA against the immunizing peptide (ab15578).

Target

Images

This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

  • ab15577 (1/50) staining Prickle in paraffin-embbeded human uterus (smooth muscle). Tissues underwent formaldehyde fixation, heat mediated antigen retrieval and peroxidase blocking prior to staining of the sections. Please refer to Abreview for further experimental details.

    See Abreview

References

ab15577 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Uterus (smooth muscle))
Specification
Uterus (smooth muscle)
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization
No
Blocking step
5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

Submitted Feb 01 2011

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Bone and muscle)
Specification
Bone and muscle
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 09 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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