All tags Primary Antibodies A comparison of leading Ki67 antibodies for IHC

A comparison of leading Ki67 antibodies for IHC

A comparison of anti ki67 antibodies for immunohistochemistry (IHC) with the leading alternatives by an independent academic collaborator.

Summary

  • Our anti-Ki67 antibodies accurately stain proliferating cells in tissue sections
  • They produce less background and can be used at much lower concentrations than the leading alternatives
  • We have knockout validated our anti-Ki67 antibodies used here to ensure specificity


The figures below are serial sections of human tonsil. IHC was carried out on paraffin-embedded sections following heat-mediated antigen retrieval using citric acid. Blocking was with 1% bovine serum albumin (BSA). The secondary antibody was conjugated to biotin (ab207995) (1:300 dilution) and detected with streptavidin conjugated to DAB.


Polyclonal antibodies


AB
AntibodyLeading anti-Ki67 polyclonalAnti-Ki67 antibody (ab15580)
Knockout validated?NoYes
Optimal dilution1:1001:600
Staining patternLow specificity with high backgroundSpecific with low background
VerdictPoorGood


Monoclonal antibodies


AB
AntibodyLeading anti-Ki67 monoclonalAnti-Ki67 antibody [SP6] (ab16667)
Knockout validated?NoYes
Optimal dilution1:2001:1,000
Staining patternSpecific with very low backgroundSpecific with very low background
VerdictExcellentExcellent


View the datasheets for our anti-Ki67 antibodies (ab15580 and ab16667) tested here

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