All tags RabMAb Anti-PEG RabMAb® products for pharmacokinetics

Anti-PEG RabMAb® products for pharmacokinetics

Pharmacokinetics is the mathematics of the time course of absorption, distribution, metabolism, and excretion of drugs in the body. In an effort to examine the effectiveness of Anti-PEG RabMAb primary antibodies to track the metabolism rate of a PEGylated antigen within an animal model, the following experiment was performed.

1. A rat was injected intraperitineally using 100ug of
    mouse IgG-PEG as an antigen.

2. Blood was drawn at 0.5, 1, 2, 4, 10, 24 and 48 hours post-injection.

3. The antiserum was extracted and tested for IgG concentrations
    using Anti-PEG RabMAb primary antibody in an ELISA assay.

4. In a 96-well plate coated with 5ug/mL of Anti-PEG RabMAb primary
    antibody (
ab51257, clone: PEG-B-47),  PEG-HRP and the antiserum
​    were added. 

5. Using quantified IgG concentrations from the rat antiserum along with
    a standard curve generated from an IgG-PEG + HRP-PEG competitive
    assay (Fig. 1), the concentration of injected mouse IgG-PEG over time
    can be determined (Fig. 2).

As demonstrated below, Anti-PEG RabMAb primary antibodies can be a used to accurately quantify targets of interest and investigate the pharmacokinetcs of PEGylated drugs or antigens within a system (Fig. 2).

Fig 1. Mouse IgG-PEG and PEG-HRP competitive ELISA assay using
varying amounts of IgG-PEG and PEG-HRP at a dilution factor of
1:30,000.

Fig 2. Mouse IgG-PEG concentration (ug/mL) detected in antiserum
at times points (h) post injection using 5 ug/mL Anti-PEG RabMAb primary antibody (ab51257, clone: PEG-B-47)