All tags Custom Services Developing custom rabbit monoclonal antibodies against cleavage specific epitopes

Developing custom rabbit monoclonal antibodies against cleavage specific epitopes

Protein cleavage and proteolysis play major roles in diverse biological processes including:

Normal protein processing and maturation

  • Regulation of protein activity
  • Protein localization to different cellular compartments
  • Regulation of intracellular protein levels

Site-specific cleavage often results in protein fragments that possess distinct functional properties. The study of protein cleavage is greatly enhanced by cleavage specific antibodies that offer a simple and sensitive way to detect and quantify the cleaved fragments.

Rabbits have a unique immune system which allows them to develop antibodies that recognize subtle changes in epitopes such as those created upon protein cleavage. The high specificity and affinity of RabMAb® advantages allow unequivocal resolution of parental and cleaved protein fragments in Western blot, FACS, and IHC applications. 

Our Custom Services team has a demonstrated record of success developing custom RabMAb primary antibodies that can differentiate between subtle structural variations. An example is documented in this reference, which describes anti-Amyloid beta (Abeta40 and Abeta42) antibodies developed using our RabMAb technology. 

A comparison of specificity of various PARP RabMAb primary antibodies 

Each Poly (ADP-ribose) polymerase (PARP-1) specific RabMAb primary antibody (ab32064, ab32561 and ab32138) recognizes one cleavage form with no cross-reactivity.

Anti-Caspase-3 RabMAb primary antibodies

One RabMAb primary antibody (ab32499only detects pro-form of Caspase 3 and does not recognize any cleaved caspases, while the other (ab32042) only detects the active, cleaved form of Caspase 3 and does not recognize the pro-form.