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At the same time we have observed that RabMAb primary antibodies bind to their target with greater affinity enabling higher signal-to-noise ratio than mouse mAbs at a given concentration of antibody (1).
The benefit of this is that RabMAb primary antibodies typically provide more specific and sensitive detection of their target protein with low background.
"We have spent the last year buying AGR2 antibody from various companies and sometimes from the same company with different lot numbers, searching for an antibody that does not cross react with AGR3 and therefore is specific for AGR2, does not give us background when developing western blots, does not give us background in negative control when looking at immunofluorescent staining in tissue, and of course works well for both western blot and IHC.
We have finally found it!!"
Researcher, University of Southern California
A: CD38 - FACS on HuT-78 cells using anti-CD38 RabMAb primary antibody (red) (ab108403) and a rabbit IgG (negative) (green).
B: Ki67 (Alexa Fluor® 488) - IF on HeLa cells using anti-Ki67 RabMAb primary antibody (ab154201).