Directly conjugated antibodies for immunofluorescence

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All tags RabMAb Low background: the first RabMAb® advantage

Low background: the first RabMAb® advantage

As monoclonals, RabMAb primary antibodies detect a single epitope so are less likely to cross-react with other proteins.

At the same time we have observed that RabMAb primary antibodies bind to their target with greater affinity enabling higher signal-to-noise ratio than mouse mAbs at a given concentration of antibody (1).

The benefit of this is that RabMAb primary antibodies typically provide more specific and sensitive detection of their target protein with low background.

"We have spent the last year buying AGR2 antibody from various companies and sometimes from the same company with different lot numbers, searching for an antibody that does not cross react with AGR3 and therefore is specific for AGR2, does not give us background when developing western blots, does not give us background in negative control when looking at immunofluorescent staining in tissue, and of course works well for both western blot and IHC.

We have finally found it!!"

Researcher, University of Southern California 

Low background results in a variety of applications and cell types or tissues

c-myc IHC ab32072 and AMPK-alpha-1 IF ab92701

A: C-myc  -  IHC on FFPE human colonic adenocarcinoma using anti-c-Myc RabMAb primary antibody (ab32072).

B: AMPK alpha 1 (phospho S496) - IF on HeLa cells using anti-Phospho-AMPK alpha 1 (pS496) RabMAb primary antibody (ab92701).


MCM2 WB ab108935 and histone h3 IF ab32107

A: MCM2 - WB on (1) MCF-7, (2) Ramos, (3) SW480, (4) Molt-4, (5) Jurkat, and (6) HeLa cell lysates using anti-MCM2 RabMAb primary antibody (ab108935).

B: Histone H3 (phospho S10) - IF on HeLa using anti-Histone H3 (phospho S10) RabMAb primary antibody (ab32107).


CD38 FACS ab108403 and Ki67 IF ab154201

A: CD38 - FACS on HuT-78 cells using anti-CD38 RabMAb primary antibody (red) (ab108403) and a rabbit IgG (negative) (green).

B: Ki67 (Alexa Fluor® 488) - IF on HeLa cells using anti-Ki67 RabMAb primary antibody (ab154201).


References

  • Rabbit monoclonal antibodies: a comparative study between a novel category of immunoreagents and the corresponding mouse monoclonal antibodies . Rossi S, Laurino L, Furlanetto A, Chinellato S, Orvieto E, Canal F, Facchetti F, Dei Tos AP. [Am J Clin Pathol. 2005 Aug;124(2):295-302]