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Recombinant rabbit monoclonal antibodies available in BSA and azide-free formats for easy antibody-labeling and functional assays.
Standard RabMAb antibodies are stored in a solution containing phosphate buffered saline (PBS), bovine serum albumin (BSA), glycerol, and sodium azide.
While BSA and azide are essential components for maintaining antibody stability and preventing antibody contamination, these components can also hinder effective conjugation to certain dyes and enzymes.
BSA competes with the primary antibody to attach to the label enzyme or dye, which greatly reduces the conjugation efficiency. The presence of sodium azide in antibody solution can be toxic to cells, limiting the antibody's use in cell culture. In addition, sodium azide can interfere with antibody conjugation and inhibits the activity of horseradish peroxidase (HRP).
Certain recombinant rabbit monoclonal antibodies have now been reformulated to overcome these problems.
PBS-only RabMAb antibodies are
ab209897: Recombinant rabbit monoclonal anti-Ki67 antibody (green), staining Ki67 in wild-type HAP1 cells (top panel) and Ki67-knockout HAP1 cells (bottom panel).
ab199376: Rabbit monoclonal IgG - Isotype control (black) utilized as a negative control in flow cytometry analysis of fixed Jurkat cells. ab199376 shows no signal overlap.